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Plasmid#80594PurposeExpresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertGFP
UseAAVTagsExpressionMammalianMutationInserted 30 nt hairpin which is targeted by Csy4 …PromoterCBAAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHL 651
Plasmid#53016PurposeLacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).DepositorInsertsLacIq
tetR
UseSynthetic BiologyTagsRBS(st3) and T1 terminatorExpressionBacterialMutationPromoterpConAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-mTAZ-WW (86)
Plasmid#40915DepositorInsertTAZ (Wwtr1 Mouse)
UseTagsGSTExpressionBacterialMutationContains mTAZ 14-3-3 binding site and WW domain (…PromoterTacAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-G51PEKN
Plasmid#118582PurposeBacterial expression of E.coli DHFR G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationG51 mutated to P51; Glu-Lys-Asn residues inserted…PromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd SUP
Plasmid#115489PurposeCRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHL 1277
Plasmid#53017PurposeAmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1DepositorInsertsGFP
mCherry
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_h7SK
Plasmid#102846PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_h7SK
UsePcr template for stagr insertsTagsExpressionMutationPromoterH7SKAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG302 5aa SunTag VP64 g4+g17 (FWA)
Plasmid#119672PurposeCRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAsDepositorInsertg17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-Y100F
Plasmid#110826PurposeBacterial expression of E.coli DHFR Y100F mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationY100 mutated to F100PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
(PD-S608A)FLAG-Cep215-FL
Plasmid#106905PurposeExpresses murine non-phosphorylatable Cep215 at S608DepositorInsertCEP215 (Cdk5rap2 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine-608 to AlaninePromoterCMVAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-mTAZ-WW (43)
Plasmid#40916DepositorInsertTAZ (Wwtr1 Mouse)
UseTagsGSTExpressionBacterialMutationContains mTAZ WW domain (43 amino acids, 122-164)PromoterTacAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
HOXB13 (human) HIS-tag pET
Plasmid#8543DepositorInsertHOXB13 (HOXB13 Human)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by Western and by transcription/tra…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
RAB14 3'UTR D1D2
Plasmid#61492Purposeluciferase reporter containing RAB14 3' UTR with both predicted MiR144 binding site deletedDepositorInsertRAB14 3' UTR (RAB14 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains position 837-1637 (numbering relative to…PromoterPGKAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 1278
Plasmid#53018PurposeAmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1DepositorInsertsmCherry
GFP
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS34-Pleum
Plasmid#60334PurposeContains a promoter of two gal binding sites coupled wiht a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGTRm
Plasmid#127197PurposepGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequencesDepositorInserttRNA-gRNA PCR template
UseTemplate plasmid for generation of golden gate pc…TagsExpressionMutationPromoterNoneAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgLacZ-1
Plasmid#74968PurposesgLacZ-1, puromycin selectionDepositorInsertLacZ
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgLacZ-2
Plasmid#74969PurposesgLacZ-2, puromycin selectionDepositorInsertLacZ
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEG302 5aa SunTag VP64 gRNA4 (FWA)
Plasmid#115481PurposeCRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNADepositorInsertg4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS12-Pleum
Plasmid#60326PurposeContains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits