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Plasmid#177576PurposeExpresses SOUL in neuronal cell typesDepositorInsertSOUL
UseAAVTagstdTomatoExpressionMutationPromoterhSynAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6
Plasmid#192184PurposeClone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6DepositorInsertClone 6 heavy chain (HH06)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2
Plasmid#192183PurposeClone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2DepositorInsertClone 2 heavy chain (HH02)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHIV-INK4A-zsGreen
Plasmid#110728PurposeMammalian Expression of p16INK4A IRES zsGreenDepositorInsertp16INK4A IRES zsGreen (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-AktDN
Plasmid#53597Purposelentiviral expression of dominant negative mouse Akt1 and EGFPDepositorInsertAkt1 (Akt1 Mouse)
UseLentiviralTagsHA and IRES-EGFPExpressionMammalianMutationDominant negative (K179M)PromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
H3S10ph biosensor
Plasmid#120809PurposeFRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRETDepositorInsertMouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
UseTagsExpressionMammalianMutationThreonine 3, Threonine 6, and Threonine 11 are mu…PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-SOUL-P2A-tdTomato-WPRE-BGHpA
Plasmid#177577PurposeExpresses SOUL in Cre-positive cellsDepositorInsertSOUL
UseAAVTagstdTomatoExpressionMutationPromoterEF1aAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-A3A(N57Q)-BE3
Plasmid#158157PurposeT7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLSDepositorInsert6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
UseTags6xHis tag on N-terminusExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPbcas13b
Plasmid#89906PurposeBacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.DepositorInsertCas13b
UseTagsExpressionBacterialMutationPromoterLacAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV_NLS-SaCas9-NLS-VPR
Plasmid#68496PurposeAAV vector containing SaCas9 fused to VPRDepositorInsertSaCas9-VPR
UseAAV and CRISPRTagsVPRExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
plko-shmfn2-mfn2-Rescue
Plasmid#160655Purpose"Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"DepositorInsertmfn2 (MFN2 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-mRuby3-WPRE
Plasmid#107744PurposeCan be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.DepositorInsertmRuby3
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-cytoBirA-2A-mCherry_Ras
Plasmid#80062PurposeSox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterSox10 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
xCas12i
Plasmid#195333Purposevector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized xCas12i
UseTags3xFlagExpressionMammalianMutationPromoterCBh, CMV, hU6Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIV-ARF-zsGreen
Plasmid#110729PurposeMammalian Expression of p14ARF IRES zsGreenDepositorInsertp14ARF IRES zsGreen (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-NLS-BirA-2A-mCherry_Ras
Plasmid#80067PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_12E
Plasmid#98652PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation12 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-EF1.Pro
Plasmid#79488PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoterDepositorInsertEF1 Promoter (EEF1A1 Synthetic, Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterEF1 PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-WT
Plasmid#180212PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1 x3 (CBX1 Human)
UseTags6xhis-tag and MBPExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_6E
Plasmid#98655PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation6 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only