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Showing: 5261 - 5280 of 16077 results

  3. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79889
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    79889-plasmid-map-sequence-id-150065

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  4. pcDNA5-FRT-TO-EGFP-AID

    Plasmid
    #80075
    Purpose
    Mammalian expression vector for N-terminal EGFP-AID tagging
    Depositor
    Andrew Holland
    Insert
    EGFP-AID
    Use
    Tags
    AID and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    80075-plasmid-map-sequence-id-150337

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  5. pcDNA5-FRT-TO-AID-EGFP

    Plasmid
    #80076
    Purpose
    Mammalian expression vector for C-terminal AID-EGFP tagging
    Depositor
    Andrew Holland
    Insert
    AID-EGFP
    Use
    Tags
    AID and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    80076-plasmid-map-sequence-id-150336

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  6. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

    Plasmid
    #169077
    Purpose
    Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII
    Depositor
    Sascha Martens
    Article
    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Inserts
    ATG5
    ATG7
    ATG10 (ATG10 Human)
    ATG12
    ATG16 (ATG16L1 Human)
    Use
    Tags
    GFP tag, GGlinker, TEV cleavage site. StrepII and…
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Polyhedrin promoter
    Availability
    Academic Institutions and Nonprofits only
    169077-plasmid-map-sequence-id-332050

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  7. Tubulin-C-18

    Plasmid
    #58197
    Purpose
    Localization: Microtubules, Excitation: N/A, Emission: N/A
    Depositor
    Michael Davidson
    Insert
    Tubulin (TUBA1B Human)
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pENTR-R2L3-cHA-IRESpuro-pA

    Plasmid
    #139537
    Purpose
    Provides HA protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    HA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    139537-plasmid-map-sequence-id-274676

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  9. pRS416-PHO5-GFP-Opi1 + terminator

    Plasmid
    #58836
    Purpose
    Expresses GFP-Tagged Opi1 + Terminator in yeast
    Depositor
    Tim Levine
    Insert
    Opi1p (OPI1 Budding Yeast)
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    PHO5
    Availability
    Academic Institutions and Nonprofits only

  10. mRuby2-N1 3xFLAG

    Plasmid
    #118980
    Purpose
    Red fluorescent labelling of proteins combined with FLAG epitope tag
    Depositor
    Paul Hamel
    Type
    Empty backbone
    Use
    Tags
    mRuby-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    118980-plasmid-map-sequence-id-231797

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  11. WT-eGFP

    Plasmid
    #215168
    Purpose
    Encodes the enhanced green fluorescent protein
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    WT-eGFP
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Availability
    Academic Institutions and Nonprofits only
    215168-plasmid-map-sequence-id-430164

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  12. pET28a(+)/FbGH17A

    Plasmid
    #100911
    Purpose
    Expression of the Beta-1,3-Endo-Laminarinase FbGH17A in E.coli
    Depositor
    Jan-Hendrik Hehemann
    Insert
    FbGH17A
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    100911-plasmid-map-sequence-id-193697

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  13. pET28a(+)/FbGH17B

    Plasmid
    #100912
    Purpose
    Expression of the Beta-1,3-Exo-Laminarinase FbGH17B in E.coli
    Depositor
    Jan-Hendrik Hehemann
    Insert
    FbGH17Bcat
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    not full length
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    100912-plasmid-map-sequence-id-193695

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  14. PSMA4_GFP_Cterm

    Plasmid
    #221514
    Purpose
    Fluorescently tagged Psma4-GFP
    Depositor
    Alessandro Ori
    Insert
    PSMA4 (PSMA4 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221514-plasmid-map-sequence-id-439377

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  15. HCP3

    Plasmid
    #166105
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    166105-plasmid-map-sequence-id-330319

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  20. pCas1_Cas2/3

    Plasmid
    #89240
    Purpose
    Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1
    Depositor
    Blake Wiedenheft
    Article
    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p
    Insert
    Cas1 and Cas2/3
    Use
    Tags
    Strep II tag on Cas1
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    89240-plasmid-map-sequence-id-176381

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Showing: 5261 - 5280 of 16077 results