Addgene

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pQLinkHD_EBFP2-ECFP

Plasmid

#118854

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2-ECFP

Use

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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S5_EcoRV_Ef1a_AscI

Plasmid

#128469

Purpose

Ef1a promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

Ef1a promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S11_EcoRV_CAMKIIA_AscI

Plasmid

#128475

Purpose

CAMKII1 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

CAMKII1 promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S10_EcoRV_hcSYN_AscI

Plasmid

#128474

Purpose

hcSYN promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

hcSYN promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSM002

Plasmid

#122936

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML043C (Marked)

Use

Tags

Expression

Yeast

Mutation

YML043C: bp 52-63 AAGTTAAAGTAT to AAATTGAAATAC

Promoter

[tetO2]4inPgal1

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSM001

Plasmid

#122935

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML029W (Marked)

Use

Tags

Expression

Yeast

Mutation

YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

Promoter

[tetO2]4inPgal1

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEC005

Plasmid

#122939

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML071C (Marked)

Use

Tags

Expression

Yeast

Mutation

YML071C: bp 52-63 AAACGGTTAAGT to AAGCGCTTGAGC

Promoter

[tetO2]4inPgal1

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_207

Plasmid

#122938

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML070W (Marked)

Use

Tags

Expression

Yeast

Mutation

YML070W: bp 52-63 GGGTTTGCCCTT to GGCTTCGCGCTA

Promoter

[tetO2]4inPgal1

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_243

Plasmid

#122940

Purpose

Expression of the marked mRNA under the control of insertional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML029W (Marked)

Use

Tags

Expression

Yeast

Mutation

YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

Promoter

Pyml029w-tetO(2)

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSM004

Plasmid

#122937

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML065W (Marked)

Use

Tags

Expression

Yeast

Mutation

YML065W: bp 52-63 GAGCAGGGAAAT to GAACAAGGTAAC

Promoter

[tetO2]4inPgal1

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_228

Plasmid

#122933

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YBR025C (Marked)

Use

Tags

Expression

Yeast

Mutation

YBR025C: bp 52-63 GGTAATAACTTG to GGAAACAATTTA

Promoter

[tetO2]4inPgal1

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_229

Plasmid

#122934

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YBR026C (Marked)

Use

Tags

Expression

Yeast

Mutation

YBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAG

Promoter

[tetO2]4inPgal1

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2-mTFP1

Plasmid

#118855

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2-mTFP1

Use

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBP-BBa_J23100

Plasmid

#74097

Purpose

BBa_J23100 promoter (iGEM collection)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_J23100 constitutive promoter (Sigma70)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pM2P20X

Plasmid

#108210

Purpose

Expresses MSePCNA2-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA1-PdX fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pS3C

Plasmid

#108211

Purpose

Expresses SsoPCNA3-CYP101A1 fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

SsoPCNA3-CYP101A1 fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pM3C

Plasmid

#108212

Purpose

Expresses MsePCNA3-CYP101A1 fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA3-CYP101A1 fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pM1R

Plasmid

#108208

Purpose

Expresses MsePCNA1-PdR fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA1-PdR fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pS1R

Plasmid

#108207

Purpose

Expresses SsoPCNA1-PdR fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

SsoPCNA1-PdR fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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ReNL-Actin_pcDNA3

Plasmid

#89531

Purpose

Red color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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