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Plasmid#12067DepositorInsertCUC1 mutant (CUC1 Mustard Weed)
UsePlant transformation via agrobacteriumTagsExpressionMutationmiR-resistant CUC1. Made by introducing five sile…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-PLtetO-1-UTR1-LacI-T500
Plasmid#45784DepositorInsertLac repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLtetO-1Available sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 KD ∆24-mCherry
Plasmid#80272Purposemammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP1-10 sec
Plasmid#172062PurposeUAS vector to express GFP1-10 in the ER lumenDepositorInsertGFP1-10 sec
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)
Plasmid#52906PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianMutationPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
JBEI-6833
Plasmid#84983PurposeExpression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.DepositorInsertNudB-PMD
UseTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-hygro-hPGAP3-3HA
Plasmid#50373PurposeExpress C-terminally triple HA tagged human PGAP3 in mammmalian cellsDepositorInsertPGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)
UseTags3x HAExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Control-E2F-3'UTR-Mut
Plasmid#21171DepositorInsertE2F 3'UTR Mutant (E2F1 Human)
UseLuciferaseTagsExpressionMammalianMutation2 miR binding sites mutated. Changed from GCACTTT…PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
XE28 XBC 40
Plasmid#16842DepositorInsertbeta-catenin-myc (ctnnb1.L Frog)
UseXenopus expressionTags6x mycExpressionMutationPromoterAvailable sinceMay 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
kank2_pJet1.2
Plasmid#121980Purposefor generating kank2 riboprobes for WISH in zebrafishDepositorInsertkank2 (LOC571549 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Flag-mMcl-1 KR
Plasmid#32983DepositorInsertMcl-1 (Mcl1 Mouse)
UseRetroviralTags3XFlagExpressionMammalianMutationAll Lys to ArgPromoterLTRAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6/ERGIC2-WT
Plasmid#51479PurposeExpresses ERGIC2-WT in mammalian cellsDepositorInsertERGIC2-WT (ERGIC2 Human)
UseTagsnoneExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab3 WT
Plasmid#37687DepositorInsertRab3 (Rab3 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE
Plasmid#107723PurposeFor expression of ABE7.10 in mammalian cellsDepositorInsertABE7.10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGW-dpse-GAL4-DBD
Plasmid#125153PurposeGateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CPDepositorInsertD. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
UseGateway fly expression vectorTagsExpressionInsectMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-103)
Plasmid#60357PurposeExpress the RRM1 of mouse MSI1 in bacterial cells for protein purificationDepositorInsertmouse MSI1 (7-103) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationcontains amino acids 7-103 of Msi1PromoterT7Available sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Flag Jnk3a1(2nd methione)
Plasmid#15746DepositorInsertJnk3a1-2nd M (MAPK10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-Mst1 SARAH
Plasmid#84297PurposeExpresses Mst1 SARAH domain for interaction studies.DepositorInsertMst1 (STK4 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7g2
Plasmid#14625DepositorInsertMKK7g2 (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-RAP1
Plasmid#84525PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationno stop codonPromoternoneAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only