-
Plasmid#61623PurposePlasma membrane-targeted FRET biosensor for monitoring cAMPDepositorInsertLyn-ICUE3 (RAPGEF3 Human)
UseTagsECFP, N-terminal targeting sequence from Lyn kina…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Kdm6b(1025-End)-pA
Plasmid#100278PurposeIn vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.DepositorInsertKdm6b (Kdm6b Mouse)
UseTagsFlag epitope tagExpressionMammalianMutationcatalytic domain (1025aa-End)PromoterT7Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-MAGE-2-429
Plasmid#89129Purposebacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sitesDepositorInsertMAGE-A11 (MAGEA11 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFAB815
Plasmid#47854PurposeBIOFAB reporter plasmid for measuring rnpB T1 termination efficiencyDepositorInsertrnpB T1 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO9Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo1
Plasmid#25101DepositorInsertpro-SUMO1 (SUMO1 Human)
UseTagsHisExpressionBacterialMutationChanged Serine 2 to AlaninePromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
μ2-HA-D176A
Plasmid#32754DepositorInsertμ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
UseTagsHAExpressionMammalianMutationD176APromoterCMVAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMK364 (CMV-OsTIR1-loxP-PURO-loxP)
Plasmid#121184PurposeOsTIR1 introductionDepositorInsertCMV-OsTIR1-loxP-PURO-loxP
UseCRISPRTagsExpressionMutationOsTIR1 codon optimized for human cellsPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIG2-Flag-mNuak1 WT
Plasmid#168507PurposeFor expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)DepositorInsertNuak1 (Nuak1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCAGAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-L-R279E
Plasmid#182286PurposeMammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
MmSphk2
Plasmid#118598PurposeExpresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
PXPR007 sgGFP-1
Plasmid#74960PurposeCas9 + sgGFP-1 with blasticidin selectionDepositorInsertGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
RIPR-bio-His
Plasmid#50824PurposeExpresses enzymatically monobiotinylated full length RIPRDepositorInsertRIPR
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-C7orf59
Plasmid#42336DepositorInsertC7orf59 (LAMTOR4 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMesh1
Plasmid#175594PurposeLac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
UseTagsExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 tet(A)
Plasmid#112887PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInserttet(A)
UseTags6xHisExpressionBacterialMutationSequence was codon optimized for E. coli K12 usin…PromoterPlacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-CAG-fGFP
Plasmid#108714PurposeTransposon donor plasmid for long-term expression of membrane targetted GFPDepositorInsertEGFP
UseTags6xHis and farnesylation signalExpressionMammalianMutationPromoterCAGAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIEG3-BCL6
Plasmid#40339DepositorInsertBCL6 (BCL6 Human)
UseRetroviralTagsIRES eGFPExpressionMammalianMutationoptimized translation initiation codonPromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
PERK 4: PERKKD-pGEX4T-1
Plasmid#21817DepositorInsertPERK (537-1114) (Eif2ak3 Mouse)
UseTagsGST and Myc (9E10)ExpressionBacterialMutationPERK Kinase Domain. Amino acids 537-1114.PromoterAvailable sinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro WT ATG3
Plasmid#26926DepositorInsertautophagy-related 3 (yeast) (Atg3 Mouse)
UseRetroviralTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_mU6
Plasmid#102845PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_mU6
UsePcr template for stagr insertsTagsExpressionMutationPromotermU6Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only