Addgene

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piHGpd/T101I

Plasmid

#1112

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with threonine 101 changed to isoleucine; r…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pAAV-Syn-Myr_mCherry-2A-eGFP

Plasmid

#194877

Purpose

Ratiometric Myr/Palm_mCherry control probe

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

Myristoylated/Palmitoylated_mCherry-T2A-GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-eGFP-2A-mCherry_D4H

Plasmid

#194878

Purpose

Ratiometric D4H sensor for in vivo astrocyte-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-D4H

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GfaABC1D

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-eGFP-2A-mCherry_YDA

Plasmid

#194879

Purpose

Ratiometric YDA sensor for in vivo astrocyte-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-YDA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GfaABC1D

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKDC.109

Plasmid

#227189

Purpose

T7 IPTG-driven Cas9

Depositor

Seth Shipman

Article

Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

Insert

SpyCas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTE5400_pMBP_CDS dropout vector

Plasmid

#221189

Purpose

Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

Depositor

Tobias Erb

Article

Barthel et al Synthetic Biology, 2024;, ysae013

Type

Empty backbone

Use

Tags

10x His-tag + E. coli maltose binding protein (MB…

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_NAP1-eGFP

Plasmid

#216837

Purpose

For mammalian expression of NAP1-GFP (Internal ref. SMC1638)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET-mNeonGreen-HMG-15b

Plasmid

#206872

Purpose

Express mNeonGreen-HMG

Depositor

Kyubong Jo

Article

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

mNeonGreen-HMG

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGST-EGFP-GPICEAM7

Plasmid

#213715

Purpose

Express the affinity sorting tag GST-EGFP- GPICEAM7.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGST-EGFP-GPIBY55

Plasmid

#213714

Purpose

Express the affinity sorting tag GST-EGFP- GPIBY55.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGST-EGFP-GPIDAF

Plasmid

#213713

Purpose

Express the affinity sorting tag GST-EGFP- GPIDAF.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-OptoSTIM1(CRY2clust)

Plasmid

#184715

Purpose

OptoSTIM1(CRY2clust)

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

OptoSTIM1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-EBFP2-OptoSTIM1(CRY2clust)

Plasmid

#184716

Purpose

OptoSTIM1(CRY2clust)

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

OptoSTIM1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-mTurquoise2-15b

Plasmid

#206871

Purpose

Express tTALE-mTurquoise2

Depositor

Kyubong Jo

Article

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

7-mer tTALE-mTurquoise2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-mNeonGreen-15b

Plasmid

#206870

Purpose

Express tTALE-mNeonGreen

Depositor

Kyubong Jo

Article

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

7-mer tTALE-mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-mKO2-15b

Plasmid

#206869

Purpose

Express tTALE-mKO2

Depositor

Kyubong Jo

Article

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

7-mer tTALE-mKO2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Im7 F84A

Plasmid

#201082

Purpose

Im7 F84A variant inserted into CysGA at G364 via GS linker

Depositor

Shu Quan

Article

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Im7 F84A

Use

Tags

Expression

Bacterial

Mutation

Im7 F84A

Promoter

pTrc

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Green Falcan1

Plasmid

#199705

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pR

Plasmid

#193746

Purpose

Expresses LuxR under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-

Insert

LuxR

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23102

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-EGFP

Plasmid

#196606

Purpose

Dual-Luciferase Reporter vector carrying an EGFP direct sequence

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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