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Plasmid#118055PurposeUsed for expression of sgRNA in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only
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MAVS-Pex
Plasmid#44557PurposeRetroviral vector for expression of a chimeric MAVS allele targeted to peroxisomes in mammalian cellsDepositorInsertMAVS-Pex13 chimeric protein (MAVS Human)
UseRetroviralTagsExpressionMammalianMutationcontains aa1-500 of MAVS and aa136-233 of Pex13PromoterAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pgRNA-bacteria
Plasmid#44251PurposeExpression of customizable guide RNA (gRNA) for bacterial gene knockdownDepositorInsertguide RNA (bacteria)
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-GFP (MoonTag-Nb-GFP)
Plasmid#128602PurposeExpresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and sfGFPExpressionMutationPromoterSFFVAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG14-MS2-GFP
Plasmid#27117DepositorInsertMS2-binding protein
UseTagsGFP, HA, and NLSExpressionYeastMutationPromoterAvailable sinceDec. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 WT
Plasmid#12301DepositorInsertSTAT1 (STAT1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_C
Plasmid#168050PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
UseTagsExpressionBacterialMutationPromoterJ23101**Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKV059
Plasmid#133314PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and IPTDepositorInsertLuc+, IPT
UseTagsExpressionPlantMutationPromoterAtUbi10, 35SAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA
Plasmid#20299PurposeCre-activated AAV expression of mCherryDepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1_Ec_rpoZ
Plasmid#128837PurposeE. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.DepositorInsertrpoZ (rpoZ E. coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
CS-TRE-c-Myc-Ubc-rtTA
Plasmid#198190PurposeGeneration of Expandable megakaryocyte cell lines from human iPS cellsDepositorInserthC-MYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Dnmt3a
Plasmid#84476PurposeLentiviral construct to express dCas9 fused with Dnmt3aDepositorInsertdCas9-Dnmt3a (DNMT3A Human, Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCB301-2μ-HDV
Plasmid#196329PurposeA yeast-Escherichia coli-Agrobacterium shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-WGA-mCherry
Plasmid#224119PurposeTo express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.DepositorInsertWGA-mCherry
UseAAVTagsWGA and mCherryExpressionMammalianMutationAll mutations related to the WGA in this construc…PromoterCAGIGAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Flag-c-Myc
Plasmid#102626PurposeExpresses c-Myc in mammalian cellsDepositorInsertMYC proto-oncogene, bHLH transcription factor (MYC Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-T2A-GFP
Plasmid#53190Purpose3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFPDepositorInserthumanized Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationPromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSHDY-PvanCC-mVenus_vanR
Plasmid#137664PurposeConjugative vector derived from pSHDY. Constitutive expression of the repressor vanR, as well as vanillate-inducible expression of mVenus.DepositorInsertPvanCC:mVenus
UseTagsExpressionBacterialMutationPromoterPvanCCAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUGW-VGAT-pHluorin
Plasmid#207467PurposeLentiviral expression of VGAT-pHluorinDepositorArticleInsertVGAT (Slc6a12 Rat)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only