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Showing: 5181 - 5200 of 5251 results

  1. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid
    #139997
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139997-plasmid-map-sequence-id-352446

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  2. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid
    #139996
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139996-plasmid-map-sequence-id-275405

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  3. peSCBE3-NG-Hypa

    Plasmid
    #218165
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218165-plasmid-map-sequence-id-433201

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  5. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71288-plasmid-map-sequence-id-166343

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  6. pUDP002

    Plasmid
    #103872
    Purpose
    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Inserts
    hph
    Spcas9 D147Y P411T
    Use
    CRISPR
    Tags
    NLS
    Expression
    Yeast
    Mutation
    D147Y P411T
    Promoter
    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103872-plasmid-map-sequence-id-199260

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  7. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110629-plasmid-map-sequence-id-210665

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  8. Unfused click editor construct; PCV2-ePhi29 fusion - pCMV-T7-PCV2-ePhi29 (JO1420)

    Plasmid
    #217805
    Purpose
    Unfused click editor construct expressing PCV2-ePhi29(D169A), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-linker-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217805-plasmid-map-sequence-id-432902

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  12. peSCBE3-NG-HF1-Hypa

    Plasmid
    #218166
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218166-plasmid-map-sequence-id-433270

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  13. hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Plasmid
    #154429
    Purpose
    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    Depositor
    David Liu
    Article
    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:
    Insert
    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    VRQR point mutations in SpCas9
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    154429-plasmid-map-sequence-id-304585

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  15. peSCBE3-NG-HF1

    Plasmid
    #218164
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218164-plasmid-map-sequence-id-433171

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  16. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Plasmid
    #154430
    Purpose
    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    Depositor
    David Liu
    Article
    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:
    Insert
    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    VRQR point mutations in SpCas9
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    154430-plasmid-map-sequence-id-304586

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  19. pFGC-I2Cas9

    Plasmid
    #173158
    Purpose
    Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.
    Depositor
    Piotr Ziolkowski
    Article
    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:
    Inserts
    hSpCas9
    pNAP:dsRED:tNOS
    pMAS:BAR:tMAS
    ICU2 upstream regulatory region
    Use
    Tags
    3xFLAG-NLS and NLS
    Expression
    Plant
    Mutation
    Contains a D169N mutation with no visible effect …
    Promoter
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    173158-plasmid-map-sequence-id-358594

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Showing: 5181 - 5200 of 5251 results