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Plasmid#170099PurposedeGFP reporter plasmid with GTATT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PacI
Plasmid#170100PurposedeGFP reporter plasmid with unique PacI restriction enzyme recognition siteDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEc-crRNA2
Plasmid#170089Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterJ23119Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATAAC
Plasmid#170095PurposedeGFP reporter plasmid with ATAAC PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH76
Plasmid#172144PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.2TAG, luxAB; Plpp pylS; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*, Y184*)PromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
VPS35 shRNA1
Plasmid#163858PurposeshRNA targeting the coding sequences of VPS35DepositorInsertVPS35 retromer complex component (VPS35 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
VPS35 shRNA2
Plasmid#163859PurposeshRNA targeting the coding sequences of VPS35DepositorInsertVPS35 retromer complex component (VPS35 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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SAE2-mGarnet
Plasmid#175240PurposeBacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimerDepositorInsertUBA2 (UBA2 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP
Plasmid#175236PurposeBacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycinDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP
Plasmid#175235PurposeBacterial expression and purification, high affinity SUMOylation substrateDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMbd3EosHaloTAF_Hyg
Plasmid#140958PurposeTargeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusionDepositorInsertmethyl-CpG binding protein 3 (Mbd3 Synthetic, Mouse)
UseMouse TargetingTags3xFLAG, Avi, Eos, Halo, and TEVExpressionMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pChd4EosHaloTAF_Puro
Plasmid#172099PurposeTargeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusionDepositorInsertchromodomain helicase DNA binding protein 4 (Chd4 Synthetic, Mouse)
UseMouse TargetingTags3xFLAG, Avi, Eos, Halo, and TEVExpressionMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH578
Plasmid#172162PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2_v2); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH581
Plasmid#172165PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2+C); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH767
Plasmid#172172PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.WT]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB]PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH857
Plasmid#172175PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.WTopt]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH202
Plasmid#172150PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.1TAG, luxAB; Plpp 32A; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*); 32A pylS variantPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH223
Plasmid#172151PurposepylBCD variant in pCDF backboneDepositorInsertPT7/lac (pylBCD 34B_sub-pop5); LacI
UseTagsExpressionBacterialMutationPylB(N61S, E178K, E251D) PylD(A252V)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH224
Plasmid#172152PurposepylBCD variant in pCDF backboneDepositorInsertPT7/lac (pylBCD 40C_sub-pop6); LacI
UseTagsExpressionBacterialMutationPylB(N61S, E175K, E178K)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only