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Plasmid#64905PurposeKanR + ColE1 + PLlacO-1::RBS(st7):cfp::T1 terminatorDepositorTypeEmpty backboneUseSynthetic Biology; E. coliTagsExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only
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pAC-5-G4BDMD-QFADM1
Plasmid#46094DepositorInsertG4BDMD-QFADM1
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-sfGFP(1-157)
Plasmid#220743PurposeGAL4-sfGFP(1-157) construct expression in mammlian cells.DepositorInsertGAL4-sfGFP(1-157)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbLAG16
Plasmid#220739PurposeNIR-FbLAG16 expression in mammalian cells.DepositorInsertNIR-FbLAG16
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbLAG30
Plasmid#220740PurposeNIR-FbLAG30 expression in mammalian cells.DepositorInsertNIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb(718)
Plasmid#220742PurposeNIR-Fb(718) expression in mammalian cells.DepositorInsertNIR-Fb(718)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
tRECs/3xUAS-FP attB
Plasmid#220330Purposeencodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)DepositorInsertsB3RT-STOP-B3RT-TagBFP-3xHA
KDRT-STOP-KDRT-GFP-HSV
FRTm71-STOP-FRTm71-mCherry
UseTagsExpressionInsectMutationPromoterUASAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-SpyTag003
Plasmid#216282PurposeExpresses SUMO with a C-terminal SpyTag003 in bacterial cellsDepositorInsertSUMO-SpyTag003
UseTagsHis6 and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-OmpA-NHSTIIT-GG
Plasmid#215790PurposeCloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-SufI-NHSTIIT-GG
Plasmid#215791PurposeCloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCYP31
Plasmid#212864PurposeExpresses the Saccharomonospora piscinae LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
lvl0 MethylAblet
Plasmid#153412PurposeIt is used to bypass the Level 1 cloning and directly feed terminators into Level 2DepositorInsertamilCP
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pR-AntiDsRed
Plasmid#139678PurposeCassette for testing R recombinase activity using a fluorescent reporterDepositorInsertAntiDsRed RS
UseTagsExpressionPlantMutationPromoterMaize UbiquitinAvailable sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSM034
Plasmid#128292Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPara - lp-sopB
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSPB847
Plasmid#128297Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPara - parB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSPB858
Plasmid#128300Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPara - GCN4zip.MSE-parB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSPB848
Plasmid#128299Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPara - lp-parB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM105
Plasmid#128295Purposeasymmetric plasmid partitioning E. coli - negative controlDepositorInsertPara - lp-cfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only