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Plasmid#166132PurposeFor expression of human talin-1 head fragment (residues 206-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertT1head1-F2F3(206-405) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 206-405 onlyPromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 5A
Plasmid#60651PurposeExpresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationmutated five serines to alaninesPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1
Plasmid#220610PurposeNeuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporterDepositorInsertFLAG-KORD-P2A-ArgiNLS-AausFP1
UseAAV and Cre/LoxTagsFLAG; ArgiNLSExpressionMammalianMutationPromoterhSynAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1845 - pAAV CaMKII hChR2(H134R)-V5-ER-LBD (ChRERa)
Plasmid#201822PurposeAn adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CaMKii promoter, for use in PET imagingDepositorInserthChR2(H134R)-V5-ER-LBD (ChRERa)
UseAAVTagsExpressionMutationPromoterCaMKiiAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
t1-405(del30)
Plasmid#166128PurposeExpression of human talin-1 head (residues 1-405) in E. coli. Contains 30 amino acid deletion in F1-loop. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertT1head1-405(del30) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-405, with 30 amino acid deletion in th…PromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
t1-405(T144E,T150E)
Plasmid#166130PurposeFor expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertHuT1head1-405(T144E,T150E) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-405, mutations T144E and T150EPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 4A
Plasmid#59907PurposeExpresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationmutated four serines to alaninesPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core
Plasmid#61357Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (D1399Y)
Plasmid#61358Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; D1399Y mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-C 16E7
Plasmid#37880DepositorInsertHPV 16 E7 (E7 HPV)
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceJuly 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N E4orf1
Plasmid#38063DepositorInsertE4 orf1
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N EBNA3A
Plasmid#37956DepositorInsertEBNA3A (EBNA-3A H. Herpesvirus 4 (EBV))
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N BARF1
Plasmid#37922DepositorInsertBARF1 (BARF1 H. Herpesvirus 4 (EBV))
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N E1B 19k
Plasmid#38047DepositorInsertE1B 19K
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N GFP
Plasmid#37855DepositorInsertGFP
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N 16E5
Plasmid#37874DepositorInsertHPV 16 E5 (E5 )
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceJuly 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N BALF1
Plasmid#37921DepositorInsertBALF1 (BALF1 H. Herpesvirus 4 (EBV))
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-C 18E7
Plasmid#37886DepositorInsertHPV 18 E7 (E7 HPV)
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceJuly 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-C 18E6
Plasmid#37884DepositorInsertHPV 18E6 (E6 HPV)
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceJuly 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-C 16E6
Plasmid#37876DepositorInsertHPV 16E6 (E6 HPV)
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceJuly 25, 2012AvailabilityAcademic Institutions and Nonprofits only