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Plasmid#71547Purposemammalian expression of Sept 2 with a GFP fusionDepositorInsertSeptin 2 (Septin2 Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-miniTurbo-V5_pCDNA3
Plasmid#107174Purposeexpresses V5-tagged miniTurbo on the mammalian ER membraneDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionMammalianMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHKMC1: Empty sgRNA for Cloning
Plasmid#67720PurposeEmpty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterPU6Available sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-PHYTipi
Plasmid#53283PurposeContains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2
Plasmid#87693PurposeExpress ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cellsDepositorInsertERT2-PhoCl-Gal4VP16-PhoCl-ERT2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZLCv2-3xFLAG-dCas9-HA-2xNLS
Plasmid#106357PurposeEmpty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitationDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3XFLAG and HAExpressionMammalianMutationPromoterU6Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSR40
Plasmid#69257Purposeplin-31::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterlin-31Available sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-miniTurbo-V5_pLX304
Plasmid#107176Purposeexpresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vectorDepositorInsertminiTurbo (BirA mutant)
UseLentiviralTagsExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1_SEPT2
Plasmid#71549Purposemammalian expression of Sept2 with an mCherry fusion.DepositorInsertSeptin 2 (Septin2 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-ZnT1-WT
Plasmid#82340PurposeExpresses FLAG-tagged ZnT1-WT in mammalian cellsDepositorInsertZnT1-WT (SLC30A1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.cytGAP
Plasmid#78734PurposeExpression of Ca2+ sensor GAP in the cytosolDepositorInsertcytGAP
UseTagsluciferaseExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1110
Plasmid#91031PurposeModule A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSPDepositorInsertTaCas9
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL2620 (pTniQ_Vch)
Plasmid#200892PurposeExpresses human codon-optimized V. cholerae TniQ from a CMV promoter.DepositorInsertVchTniQ
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
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PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro
Plasmid#168806PurposePiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expressionDepositorInsertASCL1-P2A-DLX2-T2A-mCherry
UseSynthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAX
Plasmid#53274PurposeContains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationDeleted idi (ORF6) and crtX genesPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-CaNAR2
Plasmid#64729PurposeCytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.DepositorInsertCaNAR2-NES
UseTagsCerulean3, Nuclear export signal (NES), and YPetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-ERK2 WT
Plasmid#8974DepositorInsertERK2 (Mapk1 Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTC217
Plasmid#70018PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1bDepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only