Addgene

We narrowed to 4,982 results for: hwa

Showing: 501 - 520 of 4982 results

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mEmerald-Sec61b-C1

Plasmid

#90992

Purpose

Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mEmerald-Sec61b (SEC61B Human, Synthetic)

Use

Tags

mEmerald (green fluorescence)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-GPAT4152-208

Plasmid

#134468

Purpose

GPAT4152-208 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

GPAT4 (GPAT4 Human)

Use

Tags

Expression

Mammalian

Mutation

GPAT4, amino acids 152-208

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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mApple-Sec61b-C1

Plasmid

#90993

Purpose

Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mApple-Sec61b (SEC61B Human, Synthetic)

Use

Tags

mApple (red fluorescence)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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Halo-p58

Plasmid

#166896

Purpose

mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

p58 (Lman1 Rat)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

L405Q, R419S- please see depositor comments

Promoter

CMV

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 delta2-29

Plasmid

#124923

Purpose

For expression of a.a.2-29 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-hsHEKsite_1_3

Plasmid

#205638

Purpose

reRNA guide targeting HEKsite_1_3

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-HEKsite_1_3 (EPHA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe2-WT

Plasmid

#85845

Purpose

Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 2 homology arms (SNCA Human)

Use

Tags

-

Expression

Bacterial and Mammalian

Mutation

-

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag Mut1-2

Plasmid

#124918

Purpose

For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L and W272F/W274F mutant

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 delta69-97

Plasmid

#124924

Purpose

For expression of a.a.69-97 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 NCD

Plasmid

#124927

Purpose

For expression of NCD (a.a. 1-139) of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-A53T

Plasmid

#85848

Purpose

Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFP

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-cyto-GCaMP6f (AAV5)

Viral Prep

#52925-AAV5

Purpose

Ready-to-use AAV5 particles produced from pZac2.1 gfaABC1D-cyto-GCaMP6f (#52925). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-cyto-GCaMP6f plasmid DNA. gfaABC1D-driven (similar to GFAP promoter) cyto-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Baljit Khakh

Article

Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

Promoter

Tags

None

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only
pTagGFP2-N SIN1 deltaCRIM

Plasmid

#124921

Purpose

For expression of Conserved Region In Middle deletion mutant of SIN1-GFP (delta139-267)

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 deltaPH

Plasmid

#124922

Purpose

For expression of Pleckstrin Homology domain deletion mutant of SIN1-GFP (delta376-486)

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-vioABE-4A6-vioC

Plasmid

#73439

Purpose

Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE-P4A6-vioC

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PG6 and P4A6 (orthogonal T7-lac variants)

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSS02:cyt-Peredox-mCherry_DS

Plasmid

#161747

Purpose

cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

Depositor

Markus Schwarzländer

Article

Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

Insert

cyt-Peredox-mCherry_DS

Use

Tags

Expression

Plant

Mutation

amino acid substitution D115S in first T-Rex doma…

Promoter

Ubiquitin 10

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-mmDNMT1

Plasmid

#205635

Purpose

reRNA guide targeting Mus Musculus DNMT1

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-mmDNMT1 (Dnmt1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 delta95-114

Plasmid

#124925

Purpose

For expression of a.a.95-114 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 delta116-127

Plasmid

#124926

Purpose

For expression of a.a.116-127 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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