-
Plasmid#138226PurposeExpresses the split mCherry reporter interrupted by the Cro-RPB2 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pFP.R357
Plasmid#138227PurposeExpresses the split mCherry reporter interrupted by the Cro-Top2 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R358
Plasmid#138228PurposeExpresses the split mCherry reporter interrupted by the CIV-RIR1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R359
Plasmid#138229PurposeExpresses the split mCherry reporter interrupted by the CP-Thy1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R361
Plasmid#138231PurposeExpresses the split mCherry reporter interrupted by the BsuP-RIR1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
AP553‐1
Plasmid#87240Purposegtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)DepositorInsertgtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
TOPO HRE
Plasmid#68458PurposeTOPO construct expressing HRE for generation of GMAP-compatible promoterDepositorInsertHypoxia Response Element
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
C39m
Plasmid#120972PurposeMoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.DepositorInsertccdB toxin
UseSynthetic BiologyTagsExpressionMutationBsaI site removedPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C64m
Plasmid#120980PurposeMoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).DepositorInserttemperature sensitive lambda repressor (cI857)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C35m
Plasmid#120969PurposeMoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).DepositorInsertRhlR
UseSynthetic BiologyTagsExpressionMutationBsaI and BbsI sites removed; amino acid sequence …PromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
UC20m
Plasmid#121040PurposeMoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).DepositorInsertgN2 Cas9 gRNA UC part
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C71m
Plasmid#120983PurposeMoClo golden gate assembly CD part for CinR-CIDDHYRTC n-2 (Adam Meyer's improved CinR activator from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).DepositorInsertCinR Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBP-TagR_MCD22
Plasmid#204104PurposeType 0 tag-compatible RBS partDepositorInsertMCD22 weak RBS for assembly with tag part
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-TagR_MCD8
Plasmid#204105PurposeType 0 tag-compatible RBS partDepositorInsertMCD8 weak RBS for assembly with tag part
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-TagR_MCD10
Plasmid#204101PurposeType 0 tag-compatible RBS partDepositorInsertMCD10 medium RBS for assembly with tag part
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-TagR_MCD7
Plasmid#204102PurposeType 0 tag-compatible RBS partDepositorInsertMCD7 medium RBS for assembly with tag part
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-TagR_MCD15
Plasmid#204103PurposeType 0 tag-compatible RBS partDepositorInsertMCD15 medium RBS for assembly with tag part
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-TagR_MCD2
Plasmid#204098PurposeType 0 tag-compatible RBS partDepositorInsertMCD2 strong RBS for assembly with tag part
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-TagR_MCD1
Plasmid#204099PurposeType 0 tag-compatible RBS partDepositorInsertMCD1 strong RBS for assembly with tag part
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-TagR_MCD17
Plasmid#204100PurposeType 0 tag-compatible RBS partDepositorInsertMCD17 medium RBS for assembly with tag part
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only