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Plasmid#113456PurposeLentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseLentiviralTagsProteinA-mCitrineExpressionMammalianMutationPromoterhUbCAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only
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pLenti PA-mCit
Plasmid#113451PurposeLentiviral ProteinA-mCitrine expression vectorDepositorInsertProteinA-mCitrine
UseLentiviralTagsExpressionMammalianMutationPromoterhUBbCAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 14 mCit mCer
Plasmid#83565PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.DepositorInsertPKC alpha (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSN25
Plasmid#100110Purposeexpresses a chimeric SOX2-repressor fusionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti PA-mCit-NL
Plasmid#113452PurposeLentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)DepositorInsertProteinA-mCitrine-NanoLuc
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterhUBbCAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoFGFR1-Y766F
Plasmid#59777PurposeExpresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1DepositorInsertOptoFGFR1-Y766F (FGFR1 Human, Mustard Weed)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoFGFR1-D387A
Plasmid#59778PurposeExpresses optoFGFR1 with mutation in PHR to suppress homointeraction, thus cannot be activated by lightDepositorInsertOptoFGFR1-D387A (FGFR1 Human, Mustard Weed)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-mCit-BAD
Plasmid#113459PurposeProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)DepositorInsertBCL2 associated agonist of cell death (BAD Human)
UseTagsProteinA-mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB5318
Plasmid#204828PurposeAn integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetS promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB5321
Plasmid#204831PurposeAn integrating plasmid containing both the inducible enotetSW3 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW3 promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB5320
Plasmid#204830PurposeAn integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW2 promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB5322
Plasmid#204832PurposeAn integrating plasmid containing both the inducible enotetSW4 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW4 promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB5319
Plasmid#204829PurposeAn integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW1 promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionRD
Plasmid#172924PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorRD
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionRE
Plasmid#172926PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorRE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionKE
Plasmid#172925PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorKE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_crGE
Plasmid#172933PurposeCrowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crGE
Plasmid#172927PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ionRD
Plasmid#172931PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.DepositorInsertIonicStrengthSensorRD
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ionRE
Plasmid#172932PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.DepositorInsertIonicStrengthSensorRE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only