Addgene

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pminiT-MED6-Halo donor

Plasmid

#197866

Purpose

Donor plasmid used for building MED6-Halo knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

Med6 (Med6 Mouse)

Use

Mouse Targeting

Tags

Halotag

Expression

Mutation

Promoter

NA

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX330_ATP1A1_G7

Plasmid

#173204

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + SpCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-SARA-V5-His6

Plasmid

#67431

Purpose

Expression of hSara with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
M-R742X (BF7-2)

Plasmid

#21372

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type II (PKD2 Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

changed R742 to stop codon

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pET-DEST42-SAR1-V5-His6

Plasmid

#67430

Purpose

Expression of hSar1 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAJ01

Plasmid

#14908

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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mP14H1

Plasmid

#84369

Purpose

antigen for mouse PRDM14 purification ( aa 1-231)

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14

Use

Tags

6xHis

Expression

Bacterial

Mutation

only aa 1-231 present

Promoter

T7

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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hP14H8y

Plasmid

#84368

Purpose

antigen for human PRDM14 purification ( aa 1-243)

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

contains PRDM14 AA1-243

Promoter

T7

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAL942

Plasmid

#119721

Purpose

precursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 Cysteine

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

precusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C

Use

Tags

none

Expression

Bacterial

Mutation

Alanine 248 to Threonine, Alanine 188 to Cysteine…

Promoter

T7

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST14-ARL5B

Plasmid

#67379

Purpose

Expression of native hArl5b in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5B (ARL5B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-SARA-V5-His6

Plasmid

#67452

Purpose

Mammalian expression of hSara with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAJ02

Plasmid

#14909

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

Plasmid

#195567

Purpose

PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

TIR1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1-alpha

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF18_TurboID_3xHA

Plasmid

#200017

Purpose

Expresses KSHV protein ORF18 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF18

Use

Tags

TurboID-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF30_TurboID_3xHA

Plasmid

#200018

Purpose

Expresses KSHV protein ORF30 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF30

Use

Tags

TurboID-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF31_TurboID_3xHA

Plasmid

#200019

Purpose

Expresses KSHV protein ORF31 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF31

Use

Tags

TurboID-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF66_TurboID_3xHA

Plasmid

#200020

Purpose

Expresses KSHV protein ORF66 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF66

Use

Tags

TurboID-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_3xHA_TurboID_ORF34

Plasmid

#200021

Purpose

Expresses KSHV protein ORF34 fused at the N-terminal to 3xHA-TurboID

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF34

Use

Tags

3xHA-TurboID

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-ORF29

Plasmid

#200028

Purpose

Lentiviral vector for expression of KSHV ORF29

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF29

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pminiT-MED6-mEmerald donor

Plasmid

#197865

Purpose

Donor plasmid used for building MED6-mEmerald knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

Med6 (Med6 Mouse)

Use

Mouse Targeting

Tags

mEmerald

Expression

Mutation

Promoter

NA

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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