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Plasmid#191492PurposeGreen fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoterDepositorInsertK-SPOTIT(R271F)
UseAAVTagsExpressionMutationR271F in ORPromoterCAGAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-M-SPOTIT2(R279F)
Plasmid#191493PurposeGreen fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoterDepositorInsertM-SPOTIT2(R279F)
UseAAVTagsExpressionMutationR279F in ORPromoterCAGAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-K-red-SPOTIT(R271F)
Plasmid#191494PurposeRed fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoterDepositorInsertK-red-SPOTIT(R271F)
UseAAVTagsExpressionMutationR271F in ORPromoterCAGAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-M-red-SPOTIT2(R279F)
Plasmid#191495PurposeRed fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoterDepositorInsertM-red-SPOTIT2(R279F)
UseAAVTagsExpressionMutationR279F in ORPromoterCAGAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
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TFORF1059
Plasmid#143914PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFATC1 (NFATC1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
AK7 gRNA (BRDN0001146118)
Plasmid#77914Purpose3rd generation lentiviral gRNA plasmid targeting human AK7DepositorInsertAK7 (AK7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96)
Plasmid#50914PurposeExpresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cellsDepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and YFPExpressionMammalianMutationP676C I730C N-term deletion of aa13-222 in hNKCC1…PromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFD5
Plasmid#73914Purposemultiplex gRNA plasmidDepositorInsertdU6-3:tRNA:gRNA:tRNA:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tom20-V5-FKBP-AP_pLX304
Plasmid#120914PurposeLentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membraneDepositorInsertTom20-V5-FKBP-AP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgRosa26
Plasmid#159914PurposeMutagenesis of Rosa26 with SauCas9DepositorInsertRosa26 gRNA (Gt(ROSA)26Sor Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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nanobody(AntiGFP)-HaloTag-iLID
Plasmid#191453PurposeLocalizing iLID with GFP-tagged proteinsDepositorInsertnanobody(AntiGFP)-HaloTag-iLID
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac-Giardia Dicer
Plasmid#89146PurposeExpression of Giardia Dicer in Sf9 cellsDepositorInsertGiardia Dicer
UseTagsHis6-teveExpressionInsectMutationPromoterAvailable sinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pGP-20XUAS-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10
Plasmid#172914PurposeGal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP7c-T2A-tdTomato
UseTags6xHis and T2A-tdTomatoExpressionInsectMutationPromoterhsp70Available sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4134339
Plasmid#152914PurposeMammalian expression plasmid for SARS-CoV-2 endoRNAseDepositorInsertSARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TapTagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046537
Plasmid#153914PurposeYeast expression plasmid for SARS-CoV-2 endoRNAseDepositorInsertSARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046635
Plasmid#149149PurposeYeast Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits