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Plasmid#158445PurposeGateway Cloning compatible entry vector for the human CTSS gene.DepositorInsertCTSS (CTSS Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJWK_VD_02
Plasmid#158103Purpose5'UTR stem-loop luciferase reporter: -30kcal/molDepositorInsert5'UTR synthetic stem-loop -30kcal/mol
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8.5
Plasmid#158407PurposeExpress single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_S.C.V5_NGFR
Plasmid#158331PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsS.C.V5ExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold-CAF1-C-10
Plasmid#158003PurposeVisualization of nucleus in mammalian cells using mGoldDepositorInsertCAF1 (Chaf1a Mouse)
UseTagsmGoldExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAS-Ceru-10unit
Plasmid#158389Purpose10-unit intMEMOIR recording array for Drosophila melanogasterDepositorInsert10unit recording array
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
-2701 hTSP2 promoter Luciferase
Plasmid#15816DepositorInsertTSP2 promoter (THBS2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLKO_V5.AU1.FLAG_NGFR
Plasmid#158240PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsV5.AU1.FLAGExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-PHKG2_IDG-K
Plasmid#158186PurposeGateway destination clone of PHKG2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-PHKG2 (PHKG2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HSV.Ollas.FLAG_NGFR
Plasmid#158270PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHSV.Ollas.FLAGExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
M10.2-IRES-tauVenus-ACNF Targeting Vector
Plasmid#15858DepositorInsertM10.2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO_NWS.V5.VSVg_NGFR
Plasmid#158246PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsNWS.V5.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_V5.FLAG.VSVg_NGFR
Plasmid#158247PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsV5.FLAG.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_NWS.V5.FLAG_NGFR
Plasmid#158251PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsNWS.V5.FLAGExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EYFP-ccdB-EBFP2
Plasmid#158553PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGT3
Plasmid#15825DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pZA13
Plasmid#158491PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationno STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Barcode backbone
Plasmid#158665PurposeThis plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combinationDepositorTypeEmpty backboneUseLentiviralTagshcREDExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPSG465
Plasmid#158587PurposeEnzymes Bv-CYP76AD5, DODC, ARO4_K229LDepositorInsertsBv-CYP76AD5
DODC
ARO4_K229L
UseTagsExpressionYeastMutationK229LPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO_S.V5.HA_NGFR
Plasmid#158339PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsS.V5.HAExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only