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pDK365

Plasmid

#134195

Purpose

Retroviral plasmid with SKAP insert

Depositor

Iain Cheeseman

Insert

SKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)

Use

Retroviral

Tags

LAP (GFP)

Expression

Mammalian

Mutation

Alternate Start, G5 siRNA hardened

Promoter

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pH9-ilvA-ccdBwt (DE25)

Plasmid

#135170

Purpose

Expressing synthetic overlapping gene, ilvA-ccdB

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

ilvA-ccdB

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

TetO

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pH9-ilvA-acp (DE2)

Plasmid

#135163

Purpose

Expressing synthetic overlapping gene, ilvA-acpP

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

ilvA-acp

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

TetO

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBsaGSPdR

Plasmid

#108205

Purpose

PdR

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

Putidaredoxin reductase

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHPro54G

Plasmid

#108204

Purpose

4th Pro5

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

Pro-Pro-Pro-Pro-Pro

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac promoter

Available since

April 15, 2018

Availability

Academic Institutions and Nonprofits only

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LGhP-pHG165

Plasmid

#90065

Purpose

Constitutive expression of a chimeric LacI:GalR:PurR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al PLoS One. 2013 Dec 30;8(12):e83502. doi:

Insert

LGhP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-PvuRts1I

Plasmid

#86887

Purpose

express N-term His tagged PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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smtn-b

Plasmid

#78710

Purpose

Encodes zebrafish smtn-b

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

smtn-b (smtnb Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pJK502

Plasmid

#71582

Purpose

Produces Acetobacter aceti 1023 putative SixA with C-terminal His6 tag (SixAH6)

Depositor

T. J. Kappock

Article

Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

Insert

phosphohistidine phosphatase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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mEos2-Cx26-7

Plasmid

#57364

Purpose

Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Cx26

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-VASP-N-10

Plasmid

#57434

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

VASP

Use

Tags

mEos2

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pBUN82

Plasmid

#60675

Purpose

overexpression of M. smegmatis alrA gene in Mycobacteria

Depositor

Raul Barletta

Article

Caceres et al J Bacteriol. 1997 Aug;179(16):5046-55.

Insert

alrA

Use

E.coli-mycobacterium shuttle vector

Tags

Expression

Mutation

Promoter

hsp60

Available since

Dec. 23, 2014

Availability

Academic Institutions and Nonprofits only
pMP39-1

Plasmid

#45378

Depositor

Harmit Malik

Article

Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.

Insert

MAVS

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-PM mir shHoxB9 a

Plasmid

#27021

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir HoxB9 2A

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pSKB3.MPN314

Plasmid

#11433

Depositor

Sung-Hou Kim

Article

Chen et al Proteins. 2004 Jun 1. 55(4):785-91.

Insert

cell division protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pJBL2191

Plasmid

#71205

Purpose

pbuE STAR Antisense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

pbuE STAR Antisense

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

Availability

Academic Institutions and Nonprofits only

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pCAG-Noggin

Plasmid

#163586

Purpose

Expresses Noggin in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Nog (Nog Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptet2

Plasmid

#218361

Purpose

Empty backbone with tetR-PtetA expression system with two tetR operator sites

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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FUGW-PercevalHR

Plasmid

#49083

Purpose

Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_MFE1

Plasmid

#84612

Purpose

Introduces DSB at MFE1 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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