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pLKO.1P shMOCS3_2

Plasmid

#102974

Purpose

Suppression of MOCS3

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shMOCS3_2 (MOCS3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shMOCS3_1

Plasmid

#102973

Purpose

Suppression of MOCS3

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shMOCS3_1 (MOCS3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynSNAP IRES EGFP

Plasmid

#136625

Purpose

Plasmid encoding outer inner membrane localization of SNAP-Tag and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODAL-matMYC

Plasmid

#115639

Purpose

For targeted integration and inducible expression of human NODAL (with an N-terminal MYC tag on the mature peptide) using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBW829_pCAG-pExpr-LUT

Plasmid

#87554

Purpose

Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGDP3 arr

Plasmid

#112890

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

arr

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Pbla

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag PA1

Plasmid

#15560

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PA1 (PAGR1 Human)

Use

Tags

Flag and GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
BSP602

Plasmid

#122249

Purpose

expression vector for mScarlet under EFT3 Promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mScarlet

Use

Synthetic Biology

Tags

Expression

Worm

Mutation

Promoter

EFT3

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_2

Plasmid

#102964

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_2 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416T

Plasmid

#81040

Purpose

mammalian expression of LOV V416T

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2 V416T

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416T

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBW809_pCAG-PV6-F3-mCherry-BGHpA

Plasmid

#87585

Purpose

PV6 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGDP1 fosA

Plasmid

#112889

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

fosA

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Pbla

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBW820_pCAG-pExpr-FULL-ADDER

Plasmid

#87552

Purpose

Encodes full adder logic in response to Cre, Flp and VCre recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP, mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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GST-SEPT5 WT

Plasmid

#27269

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-MXC

Plasmid

#37140

Depositor

Robert Duronio

Article

White et al J Cell Biol. 2011 May 16;193(4):677-94.

Insert

Multi Sex combs (mxc Fly)

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
RV-mCD4-HH5-5

Plasmid

#124985

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-5 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-5 TCRa P2A TCRb

Use

retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab4 WT

Plasmid

#37690

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab4 (Rab4 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab35 WT

Plasmid

#37741

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab35 (Rab35 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
SEPT11 sh1 3m shRNA

Plasmid

#122021

Purpose

Negative control shRNA for SEPT11 sh1 shRNA (plasmid 122020).

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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GST-Cav (1-61)

Plasmid

#22446

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin1 (1-61)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 26, 2010

Availability

Academic Institutions and Nonprofits only