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Plasmid#187065PurposeGp41-1 Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutationDepositorInsertminiABEmax-Gp41-1
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermhCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601‐mhCMV‐ABEmaxNGA‐C2‐ E53ogRNA
Plasmid#187066PurposeGp41-1 Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutationDepositorInsertGp41-1-ABEmaxNGA
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermhCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN-R2-mCsfGFP-L3
Plasmid#178602PurposeIt can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.DepositorInsertTandem fluorescent protein timers (tFTs)
UseEntry vector for gateway cloningTagsmCherry superfolder GFPExpressionMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SHP2 D61A
Plasmid#8330DepositorInsertSHP2 (PTPN11 Human)
UseRetroviralTagsExpressionMammalianMutationD61A (activated mutant)PromoterAvailable sinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-hsDicer (Y971A/Y972A)
Plasmid#41590DepositorInserthsDicer (DICER1 Human)
UseTagsFlagExpressionMammalianMutationY971A/Y972APromoterCMVAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-dSaCas9-SID4X-pU6-sgRNA
Plasmid#158989PurposeVector F encodes pAAV-pMecp2-dSaCas9-SID4X-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neuronsDepositorInsertdSaCas9-SID4X
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterpMecp2Available sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_D27NDHFR_WTTS
Plasmid#91244Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationD27NPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YG121VDHFR_WTTS
Plasmid#91229Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31YG121VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_G121VDHFR_WTTS
Plasmid#91233Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationG121VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-HOXA1
Plasmid#47054PurposeHomeobox A1DepositorInsertHomeobox A1 (HOXA1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Miro1 K427V
Plasmid#47892PurposeExpresses myc tagged Miro1 K427 constitutively active mutantDepositorInsertMiro1 K427V (RHOT1 Human)
UseTagsmycExpressionMammalianMutationK427V, constitutively active mutantPromoterCMVAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
HisMBP CFlm25
Plasmid#78458PurposeInducible expression in E. coliDepositorInsertHuman Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
UseTagsHis and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z11
Plasmid#120054PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-iDepositorInsertmScarlet-i
UsePcr templateTagsmScarlet-iExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB161 - pL2_pSB90_2x35S::GUS::tMAS
Plasmid#123197Purposebinary plant vector for transient expression of GUS (with introns)DepositorInsert2x35S::GUS::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab7 WT
Plasmid#44824DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-hsDicer (E1564A)
Plasmid#41587DepositorInserthsDicer (DICER1 Human)
UseTagsFlagExpressionMammalianMutationE1564APromoterCMVAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIND-CLucZ
Plasmid#53224PurposeInducible expression of Cypridina luciferase and shRNAmir of interestDepositorInsertsCypridina luciferase
non-silencing shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3 Flag FKHR H215R AAA mutant
Plasmid#13510DepositorInsertFKHR H215R AAA (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationH215R T24A S256A S319APromoterAvailable sinceDec. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
SGL40C-H1.EFS.RFP657
Plasmid#69148PurposeAdvanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)DepositorInsertssgRNA
EFS
tagRFP657
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-'Q-mito
Plasmid#158764PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only