-
Plasmid#115341PurposeLiver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1DepositorInsertRIPK1 (Ripk1 Mouse)
UseAAVTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-pA-M.EcoGII
Plasmid#192873PurposepA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578DepositorInsertpA-M.EcoGII
UseTags3X Flag TagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-Bcl-XL
Plasmid#46972Purposelentiviral expression of Bcl-XLDepositorInsertBCL2L1 (BCL2L1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSI-Check2-hRluc-NFkB-firefly
Plasmid#106979PurposeLuciferase reporter with internal control for NFkB signal.DepositorInsertNFKB recognition sites (NFKB1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NEmut
Plasmid#123310PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE control sensor GRAB_NEmut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-EGFP
Plasmid#63592PurposeExpresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.DepositorInsertsCas9
EGFP
UseCRISPR and LentiviralTagsFLAG and P2AExpressionMammalianMutationPromoterEFS-NSAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-Bcl2
Plasmid#46971Purposelentiviral expression of Bcl2DepositorInsertBcl2 (BCL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)- e18 LOF
Plasmid#131729PurposeExpresses a loss of function mutant of an engineered variant of RAD18 ("e18")DepositorInserte18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
SOX17-3'gRNA
Plasmid#210465PurposegRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-inDepositorInsertSOX17 (SOX17 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX459-TP53-exon4
Plasmid#217455PurposeFor TP53 knockout targeting exon 4 of TP53DepositorInsertCRISPR Cas9 guide for TP53 (TP53 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX459-TP53-exon5
Plasmid#217456PurposeFor TP53 knockout targeting exon 5 of TP53DepositorInsertCRISPR Cas9 guide for TP53 (TP53 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-RAB10 T23N
Plasmid#130885PurposeExpresses human RAB10 T23N mutant with N-terminal GFP tagDepositorInsertRAB10 (RAB10 Human)
UseLentiviralTagsGFPExpressionMammalianMutationT23NPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA-lib 2.0
Plasmid#89638PurposesgRNA expression in mammalian cells after Gateway cloningDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE5max-SF3B1-K700E
Plasmid#200317PurposeExpresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.DepositorInsertsK700E-ng1+pg2
PEmax-2A-MLH1dn
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL123
Plasmid#187889PurposeAAVS1 safe-harbor integration of dox-inducible NFIA-SOX9DepositorInsertNFIA-SOX9
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-Akt1-K179M
Plasmid#73409PurposeExpresses kinase-inactive mutant of Akt with HA tagDepositorInsertAKT1 (AKT1 Human)
UseTagsHA tagExpressionMammalianMutationchanged K179M to make Kinase inactive mutantPromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NE1h
Plasmid#123309PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE sensor GRAB_NE1h
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL410-BRN2p
Plasmid#110733PurposeLuciferase reporter for the human BRN2 promoterDepositorInsertBRN2 promoter luciferase (POU3F2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpRYc
Plasmid#175575PurposeExpresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cellsDepositorInsertSpRYc
UseTagsEGFPExpressionMammalianMutationPID of SpRY grafted onto the N-terminal domain of…PromoterCMVAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-pro-siRNA-V2-TP53
Plasmid#111089PurposepET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.DepositorInsertTP53 (TP53 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only