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Plasmid#83157PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), T35S mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationT35SPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b K147Q
Plasmid#83155PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K147Q mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationK147QPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b K147L
Plasmid#83154PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K147L mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationK147LPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b Y64A
Plasmid#83151PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Y64A mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationY64APromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b R68S
Plasmid#83148PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), R68S mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationR68SPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b A146V
Plasmid#83147PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146V mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationA146VPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b K117N
Plasmid#83146PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K117N mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationK117NPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b M188L
Plasmid#83141PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), M188L mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationM188LPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12D/C185S
Plasmid#83165PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D/C185S mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12D/C185SPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA
Plasmid#47108PurposeExpresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertSPgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_RAD21
Plasmid#64056PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21DepositorInsertRAD21 Homology Arms (RAD21 Human)
UseTags3X Flag P2A NeoExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEntry Tshld(FKBP F36V)-GFP
Plasmid#53260PurposepEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.DepositorInsertTshld(FKBP F36V)-GFP
UseTagsEGFP, Flag, and HAExpressionMutationFKBP F36VPromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNHT7
Plasmid#186753PurposeA basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.DepositorTypeEmpty backboneUseSynthetic Biology; Cloning backboneTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_CEBPB
Plasmid#64055PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human CEBPBDepositorInsertCEBPB Homology Arms (CEBPB Human)
UseTags3X Flag P2A NeoExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_NPM1_G6
Plasmid#178091PurposeExpresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.DepositorInsertNPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDY118A
Plasmid#182958PurposepSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.DepositorInsertsCas9
Gam, Beta, Exo
I-scel
UseTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GSX2-P2A-tGFP-DTA
Plasmid#161750PurposetGFP plasmid for the c-terminal targeting of human GSX2 locusDepositorInsertturboGFP
UseCRISPRTagsP2A-tGFPExpressionMammalianMutationPromoterendogenous GSX2 promoterAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFETCH_CREB1
Plasmid#64938PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human CREB1DepositorInsertCREB1 Homology Arms (CREB1 Human)
UseTags3X Flag P2A NeoExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_LMNA_G2
Plasmid#178090PurposeExpresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.DepositorInsertLMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_GABPA
Plasmid#64253PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human GABPADepositorInsertGABPA Homology Arms (GABPA Human)
UseTags3X Flag P2A NeoExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only