Addgene

We narrowed to 11,160 results for: cel.2

Showing: 5121 - 5140 of 11160 results

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pCMV-PfV-Sapphire-IRES-DSRed

Plasmid

#116934

Purpose

Mammalian lenti vector for expression of the 40nm GEM and DSRed

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Lentiviral

Tags

Sapphire

Expression

Mutation

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRS306-PHIS3-GFP-μNS

Plasmid

#116935

Purpose

Yeast integrative vector for the expression of μNS

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

μNS

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

HIS3

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDsred monomer C1- MLPH

Plasmid

#89236

Purpose

Expression of Dsred-tagged human Melanophilin in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

Human Melanophilin (MLPH Human)

Use

Tags

Dsred monomer

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold-MannII-N-10

Plasmid

#158005

Purpose

Visualization of Golgi in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

MannII (Man2a1 Mouse)

Use

Tags

mGold

Expression

Mammalian

Mutation

Insert contains only aa 1-112 of MannII

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAV106-pTDH3-mCherry-10xSumo-6xSIM

Plasmid

#116928

Purpose

Yeast integrative vector for expression of the SUMO-SIM complex

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10xSUMO-6xSIM

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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KRAS2B

Plasmid

#55655

Purpose

expression of mouse KRAS2B

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

KRAS2B (Kras Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)

Plasmid

#194231

Purpose

Lentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a-LCB1-PDGFR TMD-T2A-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)

Plasmid

#194232

Purpose

Lentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a-LCB3-PDGFR TMD-T2A-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(48)-NLS-VSV

Plasmid

#212746

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NLS-VSV and NUbo

Expression

Yeast

Mutation

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(63)-NLS-VSV

Plasmid

#212756

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NLS-VSV and NUbo

Expression

Yeast

Mutation

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-CUP1-NUbo-E3(63) I227A-VSV

Plasmid

#212757

Purpose

Copper-inducible expression of NUbo-E3(63) I227A-VSV in budding yeast, reduced activity of E3(63)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63) I227A

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Pib1(I227A)

Promoter

CUP1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1)-CUbo-mCherry-VSV

Plasmid

#212740

Purpose

Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-mCherry-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-myc-E3(1)-CUbo-mCherry-VSV

Plasmid

#212739

Purpose

Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-mCherry-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-myc-E3(1)-CUbo-NLS-VSV

Plasmid

#212737

Purpose

Consitutive expression of myc-E3(1)-CUbo-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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iEscSnFR

Plasmid

#182807

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR

Use

Tags

6xHis, HA and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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iFluoxSnFR

Plasmid

#182808

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR

Use

Tags

6xHis, HA and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF69-2xCSTREP

Plasmid

#136230

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF69

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF69

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pFLAG-YAP1

Plasmid

#66853

Purpose

Express FLAG-tagged YAP1 in mammalian cells

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

YAP1 (YAP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pRS305-Leu2-PINO4-PfV-GS-Sapphire

Plasmid

#116930

Purpose

Yeast integrative vector for the exression of the 40nm GEM under the INO4 promoter

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Tags

Sapphire

Expression

Yeast

Mutation

Promoter

INO4

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pY002 (pFnCpf1_min)

Plasmid

#69975

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes nd artificial promoter

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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