Addgene

We narrowed to 34,820 results for: KAN

Showing: 5121 - 5140 of 34820 results

Results per page:

Sort results by:

pT2-7xTcf-NLS-ONL-CP

Plasmid

#65716

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-synDAT-S/A

Plasmid

#48792

Purpose

Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygro

Depositor

Jonathan Javitch

Article

Fog et al Neuron. 2006 Aug 17;51(4):417-29.

Insert

YFP-synhDAT-S/A (SLC6A3 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

Promoter

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-synDAT-S/D

Plasmid

#48793

Purpose

Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygro

Depositor

Jonathan Javitch

Article

Fog et al Neuron. 2006 Aug 17;51(4):417-29.

Insert

YFP-synhDAT-S/D (SLC6A3 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

Promoter

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
px335-EF-Slc2a3-R

Plasmid

#122305

Purpose

Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2-7xTcf-NLS-CNL-CP

Plasmid

#65715

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIR-31-Reporter

Plasmid

#71871

Purpose

Mammalian expression vector for the analysis of miR-31 activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-31

Use

Tags

firefly luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAL4-DBD-TBX1wt-IDR

Plasmid

#145254

Purpose

Expresses fusion of GAL4 DNA-binding domain and TBX1wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBX1wt-IDR (TBX1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM4663

Plasmid

#85126

Purpose

NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.

Depositor

Susan Golden

Article

Chang et al Science. 2015 Jul 17;349(6245):324-8. do

Insert

PkaiB-luciferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM4731

Plasmid

#85127

Purpose

NSI-NT expressing KaiB from native promoter. Tested by Topcount: Displays WT rhythms.

Depositor

Susan Golden

Article

Chang et al Science. 2015 Jul 17;349(6245):324-8. do

Insert

PkaiBC-KaiB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2-7xTcf-NLS-YNL

Plasmid

#65711

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, YNL

Use

Luciferase; Tol2 system

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Frt-V5-hspB9

Plasmid

#63110

Purpose

Expression of HSPB9 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb9 (HSPB9 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-DestFLAGHA_RBPMS2

Plasmid

#65748

Purpose

constitutively expresses FLAG-HA-tagged RBPMS2 in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
TRBV5-1

Plasmid

#123414

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV5-1 (TRBV5-1 Human)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TRAV8-1

Plasmid

#123375

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV8-1 (TRAV8-1 Human)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pN-iRS3GG

Plasmid

#98858

Purpose

Expresses pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).

Depositor

Lydia Contreras

Article

Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552

Insert

PheS cassette to be selectively replaced by unique asRNA probes

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript-Maf

Plasmid

#98179

Purpose

Expression of a cMaf in situ hybridization probe

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

cMaf, partial mRNA (Maf Mouse)

Use

In situ hybridization

Tags

Expression

Mutation

Promoter

T3/T7

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestGFP_RBPMS

Plasmid

#65744

Purpose

expresses GFP-tagged RBPMS in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoFLAG human Dendrin

Plasmid

#47934

Purpose

expresses FLAG tagged human Dendrin in mammalian cells

Depositor

Yutaka Hata

Article

Kawata et al J Biochem. 2006 May;139(5):931-9.

Insert

Dendrin-1 (DDN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

contains amino acids 2-711 of human Dendrin

Promoter

CMV

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only
sgGCAT_2

Plasmid

#72885

Purpose

CRISPR-Cas9 induced gene disruption

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

sgGCAT

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pFRT-TODestRFP_RBPMS

Plasmid

#65745

Purpose

expresses RFP-tagged RBPMS in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only