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Plasmid#163674PurposeExpresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR2-IR2 GFP pZa
Plasmid#170083PurposeGFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPdnaK-IR2-IR2Available sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-SQSfl
Plasmid#171010PurposeHiLITR protease with full-length SQS targeting information (ER)DepositorInsertEGFP-uTEV1-SQS(FL)
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_CV2
Plasmid#167165PurposepgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0193_Cq-Rpl40-Cas9
Plasmid#169346PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0193_Cq-Rpl40-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ002413Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-AARS-32
Plasmid#166540PurposescFv of a human scaffold targeting Alanyl-tRNA synthetase. Antigen coverage aa 1-968 of 968, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
hsa-cdk2 3'UTR sicheck2
Plasmid#121125Purposeluciferase assayDepositorInserthsa-cdk2 3'UTR (CDK2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-mRIPK1-S321A
Plasmid#115339PurposeMammalian expression of Flag-mRIPK1-S321ADepositorInsertRIPK1 (Ripk1 Mouse)
UseTagsFlagExpressionMammalianMutationS321APromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
UseTagsHHHHHHENLYFQGExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 S334A
Plasmid#96915PurposeMurine Atoh1 with S334A mutationDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationSerine 334 changed to AlaninePromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 S309A
Plasmid#96916PurposeMurine Atoh1 with S309A mutationDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationSerine 309 changed to AlaninePromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 S325A
Plasmid#96926PurposeMurine Atoh1 with S325A mutationDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationSerine 325 changed to AlaninePromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 S328A
Plasmid#96927PurposeMurine Atoh1 with S328A mutationDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationSerine 328 changed to AlaninePromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 S331A
Plasmid#96928PurposeMurine Atoh1 with S331A mutationDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationSerine 331 changed to AlaninePromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 S339A
Plasmid#96929PurposeMurine Atoh1 with S339A mutationDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationSerine 339 changed to AlaninePromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(WT)
Plasmid#86576PurposeExpresses cleavable human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130PromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUP95Q15ADSKGW (D7)
Plasmid#74030Purposelentiviral expression of Psd95-Q15A deltaSK-EGFP fusionDepositorInsertPsd95 (DLG4 Rat)
UseLentiviralTagsExpressionMammalianMutationQ15A, delta SH3-GK (deletion of the fragment betw…PromoterhUbCAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag-Sesn2 419A/422A/426A
Plasmid#61872PurposeLentivirial expression of Flag-Sesn 419A/422A/426A (mouse) mutantDepositorInsertSesn2 (Sesn2 Mouse)
UseTagsFlagExpressionMammalianMutation419A/422A/426APromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Zyxin-6
Plasmid#57696PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertZyxin (ZYX Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mKO-Vinculin-23
Plasmid#57852PurposeLocalization: Focal Adhesions, Excitation: 548, Emission: 561DepositorInsertVinculin (VCL Human)
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only