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Plasmid#62261Purposeexpression of A4NT sgRNA from the arabinose-inducible promoterDepositorInsertA4NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
SERA3-bio-His
Plasmid#50815PurposeExpresses enzymatically monobiotinylated full length SERA3DepositorInsertSERA3
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
SERA4-bio-His
Plasmid#50816PurposeExpresses enzymatically monobiotinylated full length SERA4DepositorInsertSERA4
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
PF13_0125-bio-His
Plasmid#50808PurposeExpresses enzymatically monobiotinylated full length PF13_0125DepositorInsertPF13_0125
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
PF14_0044-bio-His
Plasmid#50809PurposeExpresses enzymatically monobiotinylated full length PF14_0044DepositorInsertPF14_0044
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
PFA0135w-bio-His
Plasmid#50810PurposeExpresses enzymatically monobiotinylated full length PFA0135wDepositorInsertPFA0135w
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
SERA2-bio-His
Plasmid#50814PurposeExpresses enzymatically monobiotinylated full length SERA2DepositorInsertSERA2
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSRP4-bio-His
Plasmid#50806PurposeExpresses enzymatically monobiotinylated full length MSRP4DepositorInsertMSRP4
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
PFA0210c-bio-His
Plasmid#50821PurposeExpresses enzymatically monobiotinylated full length PFA0210cDepositorInsertPFA0210c
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP3.5-bio-His
Plasmid#50803PurposeExpresses enzymatically monobiotinylated full length MSP3.5DepositorInsertMSP3.5
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP8-bio-His
Plasmid#50807PurposeExpresses enzymatically monobiotinylated full length MSP8 ectodomainDepositorInsertMSP8
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
PHMcc(delta16 PHM 356s)
Plasmid#25455DepositorInsertPHM (Pam Rat)
UseTagsExpressionMammalianMutationContains the catalytic core of PHM, including aa …PromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHL 1916
Plasmid#53023PurposeAmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertfimB
UseSynthetic BiologyTagsAsp terminator and RBS(st7)ExpressionBacterialMutationPromoterpLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A3T
Plasmid#62260Purposeexpression of A3T sgRNA from the arabinose-inducible promoterDepositorInsertA3T sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A3NT
Plasmid#62259Purposeexpression of A3NT sgRNA from the arabinose-inducible promoterDepositorInsertA3NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1575
Plasmid#53028PurposePconNoHind:csrB, PLtetO-1:RBS(st3):csrDDepositorInsertscsrB
csrD
UseSynthetic BiologyTagsRBS (st3)ExpressionBacterialMutationPromoterPconNoHind and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1756
Plasmid#53036PurposePconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialMutationPromoterpConNoHind and pConNoHindM12Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)
Plasmid#225441PurposeModule for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.DepositorInserthygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5418_pMBP-GlcR
Plasmid#221192PurposeExpression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.DepositorInsertglcR (pden4400 from Paracoccus denitrificans)
UseTags10x His tag with E. coli maltose binding protein …ExpressionBacterialMutationPromoterT7 promoter and T7 promoter-lacOAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK1G3_IDG-K
Plasmid#214460PurposeGateway destination clone of CSNK1G3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1G3 (CSNK1G3 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only