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Showing: 5081 - 5100 of 30581 results

  1. Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)

    Plasmid
    #217796
    Purpose
    Variant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Cdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217796-plasmid-map-sequence-id-432863

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  2. pUC57-ITR2-H1-GFP sgRNA Merry Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210743
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 3
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 3
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210743-plasmid-map-sequence-id-420083

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  3. pUC57-ITR2-H1-GFP sgRNA Merry Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210744
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 4
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 4
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210744-plasmid-map-sequence-id-420033

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  4. pUC57-ITR2-H1-GFP sgRNA Gollum Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210747
    Purpose
    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 3
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Gollum sgRNA GFP Target 3
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    210747-plasmid-map-sequence-id-418044

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  5. pUC57-ITR2-H1-GFP sgRNA Gollum Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210748
    Purpose
    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 4
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Gollum sgRNA GFP Target 4
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    210748-plasmid-map-sequence-id-417999

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  6. pUC57-ITR2-H1-GFP sgRNA Gollum Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210746
    Purpose
    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 2
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Gollum sgRNA GFP Target 2
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    210746-plasmid-map-sequence-id-417992

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  7. pUC57-ITR2-H1-GFP sgRNA Merry Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210741
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 1
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 1
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    210741-plasmid-map-sequence-id-417990

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  8. pUC57-ITR2-H1-GFP sgRNA Gollum Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210745
    Purpose
    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 1
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Gollum sgRNA GFP Target 1
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    210745-plasmid-map-sequence-id-417963

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  9. pUC57-ITR2-H1-GFP sgRNA Merry Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210742
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 2
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 2
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    210742-plasmid-map-sequence-id-417959

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  14. pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113701
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113701-plasmid-map-sequence-id-232036

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  15. Multiple Lentiviral Expression (MuLE) system

    Plasmid Kit
    #1000000060
    Purpose
    Multiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.
    Depositor
    Ian Frew
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161928
    Purpose
    Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Insert
    Surface marker and Citrine activation reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161928-plasmid-map-sequence-id-325287

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  17. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119946
    Purpose
    Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    119946-plasmid-map-sequence-id-237566

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Showing: 5081 - 5100 of 30581 results