Addgene

We narrowed to 52,068 results for: cloning

Showing: 5061 - 5080 of 52068 results

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pICH44157

Plasmid

#50258

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, RbcS2B (AT5g38420, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL13002

Plasmid

#50266

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (0.4 kb ) + 5'UTR, 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH77901

Plasmid

#50341

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, mas (A. tumefaciens)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pME-basGal80

Plasmid

#51621

Purpose

vertebrate expression of Gal80 preceded by a basal promoter

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80 (GAL80 Budding Yeast)

Use

Vertebrate expression

Tags

Expression

Mutation

Promoter

basal promoter

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only
pICH44222

Plasmid

#50287

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR CMV1 (Cucumber Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH44188

Plasmid

#50289

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR BSMV (Barley Stripe Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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TALEN-ID12-C

Plasmid

#41477

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

Tags

3xFLAG and WT FokI nuclease domain

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_Long

Plasmid

#221471

Purpose

Gateway cloning of NKX2-1 long isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_W208L

Plasmid

#221474

Purpose

Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

W208L

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_V205F

Plasmid

#221473

Purpose

Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

V205F

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1

Plasmid

#221472

Purpose

Gateway cloning of NKX2-1 short isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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U6-BbsI-GGsite-mCherry-Puro

Plasmid

#221232

Purpose

Cloning vector for sgRNA with mCherry and Puro

Depositor

Fei Chen

Article

Chen et al bioRxiv 2024.02.01.577593

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pPRISM-QF2-he1.1:YFP

Plasmid

#184819

Purpose

Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.

Depositor

Marnie Halpern

Article

Spikol et al eLife 12:RP89516

Insert

QF2-pA-he1.1:YFP

Use

CRISPR; Backbone for cloning of homology arms (fo…

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM040_P8b_5'Ura4

Plasmid

#216461

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'ura4

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM036_P7_3"Ura4

Plasmid

#216465

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''ura4

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM024_P2_pMPA1

Plasmid

#216449

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter mpa1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM022_P2_pRPS1002

Plasmid

#216447

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rps1002

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM020_P2_pTRS1

Plasmid

#216445

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter trs1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM014_P2_pGPM1

Plasmid

#216439

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter gpm1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM042_P15678_MultiGeneBackbone

Plasmid

#216467

Purpose

Empty integration vector for genomic integration for multigene into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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