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Plasmid#71870PurposeMammalian expression vector for the analysis of miR-21 activityDepositorInsertReverse complementary sequence of miR-21
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-Blasticidin-hOCT4 WT
Plasmid#198764PurposeMammalian expression of hOCT4DepositorInserthOCT4 WT (POU5F1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
UseTagsExpressionBacterialMutationPromotertrcpAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHS1801
Plasmid#205966PurposeJAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promotersDepositorInsertCas8-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1232
Plasmid#205969PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promotersDepositorInsertCas5-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-M(BRCT)TdTwt
Plasmid#207459PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domainDepositorInsertM(BRCT)TdTwt (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA
Plasmid#176538PurposeMammalian expression of nuclear localized AwaIscBDepositorInsertAwaIscB
UseTagsNucleoplasmin NLS-3xHA and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC4-RhE-FRB-Fis1
Plasmid#68056Purposeexpress Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cellsDepositorInsertFis1 (FIS1 Human)
UseTagsFRBExpressionMammalianMutationFis1 tail (aa 93-152)PromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hPPP3CB-FLAG
Plasmid#179135PurposeExpression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-RUNX2(WT)-IDR-mCherry
Plasmid#145286PurposeBacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion ProteinDepositorInsertRUNX2(WT)-IDR (RUNX2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG wildtype, catalytic dead lipin 1
Plasmid#32006DepositorInsertLipin1 (Lpin1 Mouse)
UseTagsFLAGExpressionMammalianMutationD712E and D714EPromoterCMV, SP6Available sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-NeoR_dCas9-Sniper-KRAB_sgSTK3i_#2 (NeoR)
Plasmid#209775PurposeCRISPRi for STK3DepositorInsertdCas9-Sniper-KRAB, sgSTK3i (STK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPv2_AAVS1
Plasmid#184487PurposeLentivirus all in one CRISPR vector targeting human AAVS1 geneDepositorInsertAAVS1 (AAVS1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Tet2 promoter F
Plasmid#63879PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet2DepositorInsertTet2 Promoter fragment F
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-IRG1-N152S-HA
Plasmid#198182PurposeMammalian expression of HA-tagged human ACOD1 (IRG1) N152SDepositorInsertACOD1 (ACOD1 Human)
UseTagsHAExpressionMammalianMutationThe asparagine in IRG1 at position 152 mutates to…PromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-IRG1-H159Q-HA
Plasmid#198183PurposeMammalian expression of HA-tagged human ACOD1 (IRG1) H159QDepositorInsertACOD1 (ACOD1 Human)
UseTagsHAExpressionMammalianMutationThe histidine in IRG1 at position 159 mutates to …PromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PLEX_313 SLC31A1
Plasmid#184493PurposeLentivirus expression for SLC31A1 geneDepositorInsertSLC31A1 (SLC31A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDESTpK7WG-RPS5Ap-mito
Plasmid#163145PurposePlant mitoTALEN vector assembly, Destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoterDepositorInsertRPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG
UseTagsExpressionPlantMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Baf60c
Plasmid#72691PurposeDoxycyline regulated expression of Baf60c in mammalian cellsDepositorInsertBrg 1 (SMARCD3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Full human ELL2
Plasmid#127253PurposeExpresses full-length human ELL2 in mammalian cellsDepositorInsertELL2 (ELL2 Human)
UseOtherTagsHis6XExpressionMammalianMutationPromoterpEF1aAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only