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Showing: 5061 - 5080 of 30578 results

  2. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

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  3. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29(+exo) (LM2990)

    Plasmid
    #208958
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(M8R/V51A/M97T/G197D/E221K/…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208958-plasmid-map-sequence-id-415784

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  4. Unfused click editor construct; PCV2-EcKlenow fusion - pCMV-T7-PCV2-EcKlenow (JO1421)

    Plasmid
    #217804
    Purpose
    Unfused click editor construct expressing PCV2-EcKlenow, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-linker-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217804-plasmid-map-sequence-id-432897

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  5. Unfused click editor construct; PCV2-ePhi29 fusion - pCMV-T7-PCV2-ePhi29 (JO1420)

    Plasmid
    #217805
    Purpose
    Unfused click editor construct expressing PCV2-ePhi29(D169A), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-linker-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217805-plasmid-map-sequence-id-432902

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  7. pJEP325-pAAV-FullH1TO-SaCa9sgRNAi(CREB)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113702
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113702-plasmid-map-sequence-id-229517

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  11. R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

    Plasmid
    #170196
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)
    Depositor
    Dominic Esposito
    Article
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Insert
    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    Use
    Tags
    3C-His8-Strep2x2
    Expression
    Mammalian
    Mutation
    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…
    Promoter
    CMV51
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    170196-plasmid-map-sequence-id-334281

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  15. R620-M01-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.1.7

    Plasmid
    #170188
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)
    Depositor
    Dominic Esposito
    Article
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Insert
    SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
    Use
    Tags
    3C-His8-Strep2x2
    Expression
    Mammalian
    Mutation
    Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…
    Promoter
    CMV51
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    170188-plasmid-map-sequence-id-334256

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  16. Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)

    Plasmid
    #217802
    Purpose
    Variant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217802-plasmid-map-sequence-id-432882

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  17. Unfused click editor construct; mSA-EcKlenow fusion - pCMV-T7-mSA-EcKlenow (JO1401)

    Plasmid
    #217806
    Purpose
    Unfused click editor construct expressing mSA-EcKlenow, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217806-plasmid-map-sequence-id-432904

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  18. Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)

    Plasmid
    #217796
    Purpose
    Variant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Cdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217796-plasmid-map-sequence-id-432863

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  19. pUC57-ITR2-H1-GFP sgRNA Merry Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210743
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 3
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 3
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210743-plasmid-map-sequence-id-420083

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  20. pUC57-ITR2-H1-GFP sgRNA Merry Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210744
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 4
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 4
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210744-plasmid-map-sequence-id-420033

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Showing: 5061 - 5080 of 30578 results