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ABE8e-Cas9NG
Plasmid#203328PurposePlasmid for bacterial purification of codon optimized ABE8e-Cas9NGDepositorInsertABE8e-Cas9NG
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV-AR
Plasmid#213819PurposeLenti-viral expression of human androgen receptor wild-type (WT).DepositorInsertAR (human androgen receptor) (AR Human)
UseLentiviralTagsFlag (N terminal on backbone)ExpressionMammalianMutationNo, it is AR wild-type.PromoterEF-1aAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
evoFERNY-Cas9NG
Plasmid#203329PurposePlasmid for bacterial purification of codon optimized evoFERNY-Cas9NGDepositorInsertevoFERNY-Cas9NG
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIE
Plasmid#171083PurposeCo-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permissionDepositorUseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKM2 WT
Plasmid#107158PurposeMBP-rPKM2 WT for production and purification of rat PKM2 WTDepositorInsertMBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV2-hSyn-G-Flamp1
Plasmid#188569Purposegreen biosensor for cAMP in neuronsDepositorInsertG-Flamp1
UseAAVTagsHis tagExpressionMutationPromoterhSynAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRL_SRSF2_WT_mCherry
Plasmid#84020Purposeexpression of SRSF2 in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianMutationPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-CCND1
Plasmid#172649PurposeExpresses HA-tagged cyclin D1 in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-G-Flamp1-mut
Plasmid#188568Purposecontrol probe of G-Flamp1, insensitive to cAMPDepositorInsertG-Flamp1-mut
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
H3K9me3 biosensor
Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRRL_SRSF2_P95H_mCherry
Plasmid#84023Purposeexpression of SRSF2 mutant in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianMutationP95HPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-MCS(extended)-Puro (empty vector)
Plasmid#172603PurposeEmpty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationThe multiple cloning site (MCS) has been edited/e…PromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF6-CSPG4-myc-his
Plasmid#69037PurposeExpression of CSPG4 in mammalian cellsDepositorInsertCSPG4 (CSPG4 Human)
UseTagsHis and MycExpressionMammalianMutationnonePromoterEF-1 alphaAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1-YAP
Plasmid#166457PurposeExpresses fusion of mEGFP and YAPDepositorInsertmEGFP, YAP (YAP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1 GBP (GBP-mCherry)
Plasmid#162879PurposeExpression in mammalian cells of GFP binding protein (GBP) tagged with mCherryDepositorInsertGFP Binding Protein
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRL_SRSF2_P95L_mCherry
Plasmid#84022Purposeexpression of SRSF2 mutant in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianMutationP95LPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRL_SRSF2_P95R_mCherry
Plasmid#84021Purposeexpression of SRSF2 mutant in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianMutationP95RPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1-YAPS127A
Plasmid#166458PurposeExpresses fusion of mEGFP and YAPS127ADepositorInsertmEGFP, YAPS127A (YAP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only
