168,567 results

1. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40

   Plasmid

   #124603

   Purpose

   Cre-dependent soma-localized ChrimsonR for optogenetic stimulation

   Depositor

   Christopher Harvey

   Article

   Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

   Has Service

   AAV9

   Insert

   Soma-localized ChrimsonR
   

   Use

   AAV

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMP11

   Plasmid

   #215559

   Purpose

   pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   Cas9
   gRNA targeting pBR322 origin of replication
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23105 and tet

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-HA-mSTING

   Plasmid

   #214157

   Purpose

   Transient expression of STING

   Depositor

   Nan Yan

   Article

   Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

   Insert

   STING (Sting1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-TSF-TBK1

   Plasmid

   #216709

   Purpose

   Mammalian expression of TBK1

   Depositor

   James Hurley

   Article

   Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

   Insert

   TBK1 (TBK1 Human)
   

   Tags

   TSF

   Expression

   Mammalian

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (AAV9)

   Viral Prep

   #124603-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (#124603). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 plasmid DNA. EF1a-driven, Cre-dependent expression of ChrimsonR fused to mRuby2 fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Christopher Harvey

   Article

   Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

   Tags

   mRuby2 (Cre-dependent)

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

6. ss-cfSGFP2

   Plasmid

   #37535

   Depositor

   Ikuo Wada

   Article

   Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

   Insert

   ss-cfSGFP2 (cysteine-free SGFP2)
   

   Tags

   signal sequence of α1-antitrypsin (M1-K34)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-TFEBS211A-MYC (#805)

   Plasmid

   #99957

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

   Insert

   TFEB (TFEB Human)
   

   Tags

   6xHIs and Myc

   Expression

   Mammalian

   Mutation

   S211A

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-N1-GalT

   Plasmid

   #87327

   Purpose

   To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

   Depositor

   Lei Lu

   Article

   Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

   Insert

   1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV Retrograde)

   Viral Prep

   #162380-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   CAG

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1(+) K-Ras(G12C)

    Plasmid

    #224284

    Purpose

    Cell transfection and expression of K-Ras (G12C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    K-Ras (KRAS Human)
    

    Expression

    Mammalian

    Mutation

    G12C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

    Plasmid

    #234951

    Purpose

    Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSK-mU6-Cj-sgRNA-SV40-puro

    Plasmid

    #192128

    Purpose

    Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Cj-SgRNA scaffold
    

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

    Plasmid

    #233036

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-6xHis-MBP-TEV site-TadA8.20

    Plasmid

    #194702

    Purpose

    Expresses N-terminal MBP fused TadA8.20 in bacteria

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    TadA8.20
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    T7 promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF3358

    Plasmid

    #144834

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PEmax-P2A-BSD

    Plasmid

    #174821

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-BSD
    

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR E3 pR8A L3-L2

    Plasmid

    #158735

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NN)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    TALEN ORF without TAL repeat
    

    Use

    TALEN

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR E3 pR5A L3-L2

    Plasmid

    #158732

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last HD)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    TALEN ORF without TAL repeat
    

    Use

    TALEN

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR E1 pF8A L1-L4

    Plasmid

    #158731

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NN)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    TALEN ORF without TAL repeat
    

    Use

    TALEN

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR E1 pF5A L1-L4

    Plasmid

    #158728

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last HD)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    TALEN ORF without TAL repeat
    

    Use

    TALEN

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge