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Plasmid#193288PurposeLentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgeneDepositorInsertrtTA-P2A-tTS
UseLentiviralTagsExpressionMammalianMutationPromoterEF‐1αAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDA_479
Plasmid#179099PurposeBE Cas expressionDepositorInsertABE8e (SpG)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-mCherry FP
Plasmid#52114Purposea bicistronic IRES-mCherry containing retroviral vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES-mCherryExpressionMammalianMutationPromoterAvailable sinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_TauFRET_3xKQ_wpre
Plasmid#207522PurposeLentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cellsDepositorInsertTauFRET_P301L_3xKQ
UseLentiviralTagsNeonGreen and mTurquoise2ExpressionMutationP301L; K311Q; K317Q; K321QPromoterEF1aAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_KIAA1549_BRAF
Plasmid#205838PurposeExpress mEGFP-tagged fusion protein, KIAA1549_BRAF from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Actin-7
Plasmid#56421PurposeLocalization: Actin, Excitation: 488, Emission: 507DepositorInsertActin (ACTB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)
Plasmid#163055PurposeGrx1-roGFP2 glutathione redox sensor (cytosolic)DepositorInsertGrx1-roGFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
UPB-stomach (UPB1)
Plasmid#180784PurposeUnique Projection Barcode (UPB1)-Stomach for Projection-seqDepositorInserthumanized ChR2-H134R (partial)
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.eGFP.2A.wtTau
Plasmid#140424PurposeAAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTauDepositorInserteGFP-2A-Tau (MAPT Synthetic, Human)
UseAAVTagseGFPExpressionMutationPromoterCBAAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRx-Sth1Cas9-L5
Plasmid#140993PurposeSth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in MycobacteriaDepositorInsertsSth1Cas9
TetR
L5 attP
L5 Int
KanR
UseUnspecified; Integrative in mycobactreiaTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Neo_CAG-Cas9
Plasmid#86698PurposeCas9 expression vector for targeting to the AAVS1 locusDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterpCagAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9663 - ACTB GFP-P2A CLIP Donor
Plasmid#202688PurposeCLIP donor template for integrating GFP into ACTB locus. Encodes GFP with ACTB homology arms.DepositorInsertCopGFP
UseTagsExpressionMutationPromoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-MYOCD
Plasmid#170696Purposedoxycycline-inducible overexpression of MYOCDDepositorInsertMYOCD (MYOCD Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRMC2
Plasmid#68940Purposetetracycline-inducible expression vector for Staphylococcus aureusDepositorTypeEmpty backboneUseTet inducible e. coli/staphylococcus shuttle vect…TagsExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)
Plasmid#128164PurposeUV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialMutationPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gq-CASE
Plasmid#168125PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F124/E125 within GNAQPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGHUC w-ERBIN (2-hybrid prey plasmid)
Plasmid#128163PurposeUV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).DepositorInsertERBIN PDZ domain (ERBIN Synthetic)
UseSynthetic BiologyTagsFLAG and Omega subunit of RNA polymeraseExpressionBacterialMutationPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Connexin43-GFP-APEX2
Plasmid#49385PurposeExpresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cellsDepositorInsertConnexin43 - EGFP - APEX2
UseTagsExpressionMammalianMutationK14D, W41F, E112K, A134P on soybean APX; improved…PromoterCMVAvailable sinceNov. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOTTC476 - pAAV c-fos eYFP
Plasmid#47907PurposeAn AAV vector that expresses eYFP under the c-fos promoter.DepositorInsertenhanced Yellow Fluorescent Protein
UseAAVTagsExpressionMutationPromoterc-fosAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV MaCPNS2)
Viral Prep#37825-MaCPNS2PurposeReady-to-use AAV MaCPNS2 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. These AAV were produced with the MaCPNS2 serotype, which permits efficient transduction of the PNS in rodents (including ENS in rats) and the CNS and PNS (including ENS) in marmosets and rhesus macaques. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only