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Plasmid#154754PurposeMammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variantDepositorInsertSpike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTags2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
CAG-GFP
Plasmid#16664DepositorInserteGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-45b(+)-Tn5
Plasmid#112112PurposeExpresses hyperactive Tn5 transposase for next generation sequencing library preparationDepositorInsertTn5
UseTagsHis6ExpressionBacterialMutationE54K E110K P242A L372PPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG/GFP Asc
Plasmid#74285PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscIDepositorInsertRosa26 5-homology region
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep
Plasmid#173899PurposeThe pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThrDepositorInsertsThrRS(v1.0)
tRNA(v2.0)CUA
pduX
EF-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible mCherry reporter
Plasmid#64128PurposePhotoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoterDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterTetOx13-CMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1(Q61L)
Plasmid#13720DepositorInsertEGFP-Rac1(Q61L) (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt
Plasmid#175181PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPIDepositorHas ServiceAAV1InsertpAAV hSyn FLEX iGluSnFR3 v857.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI Anchor, IgK-chain, and Myc epi tagExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
hprtSAGFP
Plasmid#41594PurposeIn vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequencesDepositorInsertSA-GFP reporter
UseMouse Targeting; Dsb repair reporter substrateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
HIP-flash (HOP-flash mutant)
Plasmid#83466PurposeNegative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATeam1.03-nD/nA/pcDNA3
Plasmid#51958Purposemammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-LaminA-C-18
Plasmid#55068PurposeLocalization: Nuclear Envelope, Excitation: 587, Emission: 610DepositorInsertLamin A (LMNA Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Klf2-t2A-mCherry
Plasmid#127539PurposeExpresses mouse Klf2 and mCherry via t2A linker.DepositorInsertKlf2 (Klf2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-cPHx3
Plasmid#116855PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationamino acids 169-329 (tandem trimer)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO EYFP (AAV1)
Viral Prep#55641-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Flp-dependent)Available sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-Alb-mScarlet
Plasmid#183461PurposeTo produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion proteinDepositorInsertAlb (Alb Mouse)
UseAAVTagsmScarletExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.28H.28TM.28.3z
Plasmid#200678PurposeModular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IL-2 signal
Plasmid#128028PurposeMammalian expression vector with N terminal IL-2 signal peptideDepositorTypeEmpty backboneUseTagsIL-2 signal peptideExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-mEGFP
Plasmid#175293PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-EGFP
Plasmid#71666PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only