168,838 results

1. PB-tetO-hNIL

   Plasmid

   #197089

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_EF1a_Flag_TP53_WT

   Plasmid

   #183112

   Purpose

   Expresses flag-tagged p53 in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   EF1a

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. kiCAP-AAV-MyoAAV4E

   Plasmid

   #224452

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDFNNT insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJLG038

   Plasmid

   #192981

   Purpose

   BHR plasmid (pBBR1 ori) expressing T7 polymerase under control of the lac promoter

   Depositor

   Clay Fuqua

   Article

   Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

   Insert

   T7 polymerase
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM1-FIRE-pHLy

   Plasmid

   #170775

   Purpose

   Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

   Depositor

   Aimee Kao

   Article

   Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and mTFP1

   Expression

   Mammalian

   Mutation

   84 nucleotide Signal Sequence switched to the beg…

   Promoter

   CMV

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Talin-TS

   Plasmid

   #83376

   Purpose

   FRET probe for measuring tension on Talin molecule

   Depositor

   Martin Schwartz

   Article

   Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

   Insert

   Talin1 (Tln1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP-F40-tagRFP (tension sensor module)

   Expression

   Mammalian

   Mutation

   Tension sensor module is inserted between V447 an…

   Promoter

   CMV

   Available Since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt (AAV1)

   Viral Prep

   #175181-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt (#175181). In addition to the viral particles, you will also receive purified pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt plasmid DNA. Synapsin-driven, Cre-dependent, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Promoter

   Syn

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

8. AAV-U6-dgRNA-CAG-MPH

   Plasmid

   #106259

   Purpose

   Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   MS2-P65-HSF1 (HSF1 Human, Synthetic)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. XLone-ETV2

   Plasmid

   #135763

   Purpose

   expresses human ETV2 upon doxycycline treatment

   Depositor

   Xiaojun Lian

   Article

   Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

   Insert

   ETV2 (ETV2 Human)
   

   Expression

   Mammalian

   Mutation

   none

   Promoter

   TRE3G

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. v5 Cas9-eVLP

    Plasmid

    #228468

    Purpose

    Expresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag(C507V)–Cas9
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    MMLVgag(C507V)

    Promoter

    CMV

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSupAR-MbPylRS(DiZPK)

    Plasmid

    #91705

    Purpose

    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

    Depositor

    P. Chen

    Article

    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

    Inserts

    pylS
    pylT
    

    Expression

    Bacterial

    Mutation

    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pProEx-HTa-SOD1 WT

    Plasmid

    #191849

    Purpose

    express wild-type SOD1 in bacterial cells

    Depositor

    Nam-Chul Ha

    Article

    Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

    Insert

    SOD1 (SOD1 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. hNedd4-2

    Plasmid

    #83433

    Purpose

    full length Nedd4L with His Xpress tag

    Depositor

    Christie Thomas

    Article

    Itani et al Am J Physiol Renal Physiol. 2005 Aug;289

    Insert

    human Nedd4L with C2 domain (NEDD4L Human)
    

    Tags

    His Tag (6x) and Xpress Epitope Tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hsyn-GRAB_rDA1h (AAV9)

    Viral Prep

    #140557-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_rDA1h (#140557). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_rDA1h plasmid DNA. Synapsin-driven expression of red fluorescent, high affinity dopamine sensor GRAB_rDA1h. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Promoter

    Syn

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

15. HIV-1 Gag-mCherry

    Plasmid

    #85390

    Purpose

    Visualization marker for viral core to measure cellular uptake

    Depositor

    Gregory Melikyan

    Article

    Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do

    Insert

    HIV-1 gag
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLexM-SLC7A11

    Plasmid

    #197993

    Purpose

    Expresses SLC7A11 with an N-terminal FLAG tag in mammalian cells

    Depositor

    Simon Newstead

    Article

    Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

    Insert

    SLC7A11 (SLC7A11 Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. EC64420

    Plasmid

    #209517

    Purpose

    Cloning in complete guide expression cassettes, with Cas9

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TagBFP2-CAAX

    Plasmid

    #116856

    Purpose

    plasma membrane marker

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    HRAS
    

    Tags

    TagBFP2

    Expression

    Mammalian

    Mutation

    Amino acids 172-189

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK391 (mAID-BSD)

    Plasmid

    #121192

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mAID-BSD
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV.CMVenh.gp91.eGFP.cHS4

    Plasmid

    #30471

    Depositor

    Didier Trono

    Article

    Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

    Insert

    CMVenh/gp91/eGFP/cHS4
    

    Use

    Lentiviral

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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