277,000 results

1. pRK5-HA-Ubiquitin-KO

   Plasmid

   #17603

   Purpose

   Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   KO: all lysines mutated to arginines

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLCKO2::TKOv3

   Pooled Library

   #125517

   Purpose

   Human CRISPR lentiviral library containing 70,948 guides (4-5 guides/gene) targeting 18,053 human protein-coding genes, in a non-Cas9 containing backbone

   Depositor

   Jason Moffat

   Article

   Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

3. pCS2+IMS-HyPer7

   Plasmid

   #136469

   Purpose

   Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   SMAC/DIABLO

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+IMS-HyPer7

   Plasmid

   #136469

   Purpose

   Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   SMAC/DIABLO

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MZB209

   Plasmid

   #228492

   Purpose

   BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)

   Depositor

   Richard Baker

   Insert

   BirA (birA )
   

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-S5E2-GCaMP6f (AAV1)

   Viral Prep

   #135632-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Available Since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

7. TDP-43_CTD

   Plasmid

   #98669

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TDP-43_CTD

   Plasmid

   #98669

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV2-SEP-GluA1 (M1)

   Plasmid

   #64942

   Purpose

   mammalian expression of rat GluA1

   Depositor

   Jeremy Henley

   Article

   Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

   Insert

   GluA1 (Gria1 Rat)
   

   Tags

   SEP (superecliptic pHluorin)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV2-SEP-GluA1 (M1)

    Plasmid

    #64942

    Purpose

    mammalian expression of rat GluA1

    Depositor

    Jeremy Henley

    Article

    Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

    Insert

    GluA1 (Gria1 Rat)
    

    Tags

    SEP (superecliptic pHluorin)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pREDTAI

    Plasmid

    #51627

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pREDTAI

    Plasmid

    #51627

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-FLEX-ArchT-tdTomato (AAV5)

    Viral Prep

    #28305-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-FLEX-ArchT-tdTomato (#28305). In addition to the viral particles, you will also receive purified pAAV-FLEX-ArchT-tdTomato plasmid DNA. CAG-driven Cre-dependent ArchT-tdTomato expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Promoter

    CAG

    Tags

    tdTomato (Cre-dependent)

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-hSyn-hChR2(H134R)-EYFP (AAV Retrograde)

    Viral Prep

    #26973-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Syn

    Tags

    EYFP

    Available Since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

15. mEmerald-Sec61b-C1

    Plasmid

    #90992

    Purpose

    Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mEmerald-Sec61b (SEC61B Human, Synthetic)
    

    Tags

    mEmerald (green fluorescence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEmerald-Sec61b-C1

    Plasmid

    #90992

    Purpose

    Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mEmerald-Sec61b (SEC61B Synthetic, Human)
    

    Tags

    mEmerald (green fluorescence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. RHO3

    Bacterial Strain

    #124700

    Purpose

    E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

    Depositor

    Herbert Schweizer

    Article

    Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

    Bacterial Resistance

    DAP (400 µg/ml)

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

18. MLM3636

    Plasmid

    #43860

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

    Depositor

    Keith Joung

    Insert

    None
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. MLM3636

    Plasmid

    #43860

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

    Depositor

    Keith Joung

    Insert

    None
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. Human GeCKO Lentiviral sgRNA Library v2 (LentiCRISPR)

    Pooled Library

    #1000000048

    Purpose

    CRISPR gRNA knockout pooled library for targeting the human genome

    Depositor

    Feng Zhang

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only