168,897 results

1. PHYHD1

   Plasmid

   #38854

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PHYHD1 (Phyhd1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

2. Str-Golgin84_VSVG-SBP-EGFP

   Plasmid

   #65305

   Purpose

   synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCas9-T2A-GFP

   Plasmid

   #78548

   Purpose

   Cas9 protein with GFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   In frame with Cas9

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol1-U6abc_ect2_cmlc2-nmKate

   Plasmid

   #238410

   Purpose

   Drives expression of 3 different gRNAs targeting ect2, and expression of nuclear mKate in cardiomyocytes

   Depositor

   Juan Manuel González-Rosa

   Article

   Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

   Insert

   nuclear mKate/3 gRNAs targeting ect2
   

   Promoter

   cmlc2 (nmKate); U6 (gRNAs)

   Available Since

   Nov. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol1-U6abc_cmlc2(myl7)_cmlc2-nmKate

   Plasmid

   #238374

   Purpose

   Drives expression of 3 different gRNAs targeting cmlc2 (myl7), and expression of nuclear mKate in cardiomyocytes.

   Depositor

   Juan Manuel González-Rosa

   Article

   Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

   Insert

   nuclear mKate/3 gRNAs targeting cmlc2
   

   Promoter

   cmlc2 (nmKate); U6 (gRNAs)

   Available Since

   Nov. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-spCas9

   Plasmid

   #231404

   Purpose

   Expresses spCas9 from hSyn promoter

   Depositor

   Arjen Boender, Larry Young

   Article

   Schimmer et al bioRxiv 2024.07.15.603543; doi: https://

   Insert

   hSYN promoter (RIC8B Human)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   hSYN

   Available Since

   Oct. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-dCas9-BFP-KRAB

   Plasmid

   #221502

   Purpose

   CRISPRi machinery fish codon optimized

   Depositor

   Alessandro Ori

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   Fish expression

   Mutation

   D10A and H839A point mutations

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-N-Blast-sTagRFP

   Plasmid

   #207679

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-sTagRFP cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGTag Hygro EGFP nLAP -2

   Plasmid

   #194322

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(EGFP)
   

   Use

   Bacterial cloning vector

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGTag Hygro EGFP nLAP -1

    Plasmid

    #194321

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGTag Hygro EGFP nLAP -0

    Plasmid

    #194320

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGTag Hygro EGFP nLAP +1

    Plasmid

    #194306

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGTag Hygro EGFP nLAP +2

    Plasmid

    #194307

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGTag Hygro EGFP cLAP +1

    Plasmid

    #194309

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

    Insert

    cLap(EGFP)-P2A-Hygro
    

    Use

    Bacterial cloning vector

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF1a-T2-GpNLuc

    Plasmid

    #135949

    Purpose

    Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE7-P2A-BSD

    Plasmid

    #224122

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-BSD
    

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pM-Cas9

    Plasmid

    #196325

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Mutation

    See depositor comments below

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CAG-jGCaMP8s-WPRE (AAV1)

    Viral Prep

    #179256-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-CAG-jGCaMP8s-WPRE (#179256). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8s-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8s. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    CAG

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

19. Flag-APEX2-C Cloning Vector

    Plasmid

    #128146

    Purpose

    To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    Flag-APEX2

    Expression

    Mammalian

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-fDIO-Cre (AAV Retrograde)

    Viral Prep

    #121675-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Esteban Engel, Alexander Nectow

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Promoter

    EF1a

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only