168,579 results

1. pHH0103_YWHAE_14-3-3

   Plasmid

   #109942

   Purpose

   Protein expression and purification of YWHAE_14-3-3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   YWHAE_14-3-3
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHygRR2

   Plasmid

   #200075

   Purpose

   hygR selection mScarlet 2X marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Expression

   Worm

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-MBP-TEVpCS-ADGRL4 CTF-FLAG

   Plasmid

   #217709

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRL4 (ADGRL4 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-406

   Available Since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ADRB2-DuET

   Plasmid

   #213181

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADRB2 (ADRB2 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-MBP-TEVpCS-ADGRL3 CTF-FLAG

   Plasmid

   #217708

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRL3 (ADGRL3 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-841

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. ER-mNeonGreen

   Plasmid

   #137804

   Purpose

   Visualization of the Endoplasmic Reticulum

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   KDEL
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

   Plasmid

   #110854

   Purpose

   Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   sgRNA scaffold with spacer
   

   Use

   Lentiviral

   Mutation

   WT

   Promoter

   U6

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-KIR2DL5B-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156644

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR2DL5B (KIR2DL5B Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNM3689

   Plasmid

   #154111

   Purpose

   miniMos single component RMCE landing site vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
   

   Use

   Minimos

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Hyg-BFP

    Plasmid

    #214363

    Purpose

    Expression of the Blue Fluorescent Protein

    Depositor

    Bertrand Collet

    Article

    Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

    Insert

    BFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEntry_HCoV-HKU1-nsp12

    Plasmid

    #168909

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-HKU1-nsp12 (ORF1ab )
    

    Use

    Gateway-compatible entry vector

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEntry_SARS-CoV-1-nsp12

    Plasmid

    #168861

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    SARS-CoV-1-nsp12 (ORF1ab )
    

    Use

    Gateway-compatible entry vector

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ2259 - Intron GG2 - 900 bp

    Plasmid

    #159881

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG2 - 900 bp
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-AAV-sh[control]

    Plasmid

    #75438

    Purpose

    control shRNA vector

    Depositor

    Edward Burton

    Article

    Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

    Insert

    GFP
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pF CAG mc2 IRES hygro

    Plasmid

    #206234

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA6-N-3XFLAG-Gp78

    Plasmid

    #123590

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    autocrine motility factor receptor (Amfr Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mWasabi-Paxilin-22

    Plasmid

    #56509

    Purpose

    Localization: Focal Adhesions, Excitation: 493, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Paxilin
    

    Tags

    mWasabi

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. SpRY dCas9 VPR

    Plasmid

    #188511

    Purpose

    Expresses FLAG tagged SpRY dCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFJ2215 - Intron GG3 - 900 bp

    Plasmid

    #159882

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG3 - 900 bp
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pD649-HAsp-NCR2-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156567

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NCR2 (NCR2 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge