168,605 results

1. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA

   Plasmid

   #163476

   Purpose

   Direct-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpA

   Depositor

   Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   iCre
   

   Use

   AAV

   Promoter

   Beta Globin minimal promoter

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28b-BE1

   Plasmid

   #73018

   Purpose

   Expresses BE1 with N-terminal His6 tag in E. Coli

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE1
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF1337

   Plasmid

   #144171

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZBTB7A (ZBTB7A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

   Plasmid

   #112927

   Purpose

   CRISPR-a reporter assay lentiviral vector (gTetO)

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. psd44-Lactadherin46

   Plasmid

   #46830

   Purpose

   expression of human lactadherin in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   MFGE8 (MFGE8 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK promoter

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

   Plasmid

   #85452

   Purpose

   Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

   Depositor

   Aviv Regev

   Article

   Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

   Insert

   U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV5)

   Viral Prep

   #125712-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Promoter

   short version of αCamKinase II promoter (0.4kb)

   Tags

   mScarlet

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

9. pLXSN p200 CUX1

   Plasmid

   #90470

   Purpose

   Retroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectively

   Depositor

   Alain Nepveu

   Article

   Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

   Insert

   CUX1 (CUX1 Human)
   

   Use

   Retroviral

   Tags

   Ha and myc

   Expression

   Mammalian

   Promoter

   Moloney murine leukemia virus long terminal repeat

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAF0034 Ef1a-Kp03-2a-EGFP

    Plasmid

    #193461

    Purpose

    Expression of Kp03 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-SHP2 WT

    Plasmid

    #8381

    Depositor

    Ben Neel

    Insert

    SHP2 (PTPN11 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p1321 HPV-16 E6/E7

    Plasmid

    #8641

    Depositor

    Peter Howley

    Article

    Münger et al J Virol. 1989 Oct . 63(10):4417-21.

    Insert

    HPV-16 E6/E7 (E7, E6 Human papillomavirus)
    

    Expression

    Mammalian

    Available Since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HA-MBP-TEVpCS-ADGRC3 CTF-FLAG

    Plasmid

    #217686

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR3 (CELSR3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2516

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LiRTi

    Plasmid

    #214076

    Purpose

    Luminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV_hSynapsin_psychLight2 (AAV9)

    Viral Prep

    #163909-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV_hSynapsin_psychLight2 (#163909). In addition to the viral particles, you will also receive purified pAAV_hSynapsin_psychLight2 plasmid DNA. Synapsin-driven expression of the genetically encoded fluorescent sensor psychLight2 to detect behaviorally relevant serotonin release. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

    Promoter

    Syn

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

16. pDESTmycIGF2BP3

    Plasmid

    #19879

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHsp70-Cas9

    Plasmid

    #45945

    Purpose

    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Mutation

    Silent change from C to A at bp 834 of insert

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTC-ApoE-Tet

    Plasmid

    #85578

    Purpose

    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liver

    Depositor

    Ursula Ehmer

    Article

    Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

    Insert

    CreER
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    ApoE.HCR.hAAT

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-5X-1-LARP7

    Plasmid

    #113545

    Purpose

    Produces an N-terminal GST-tagged full length LARP7 protein.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    LARP7 (LARP7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti_P2A_Puro

    Plasmid

    #211364

    Purpose

    Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

    Depositor

    Jacques Neefjes

    Article

    Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge