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Addgene
Showing: 5021 - 5040 of 16077 results

  1. pET28b(+)_S. cerevisiae Hbs1(162-611)

    Plasmid
    #39337
    Depositor
    SeWon Suh
    Insert
    Elongation factor 1 alpha-like protein
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  2. pET28b(+)_S. cerevisiae Hbs1(166-611)

    Plasmid
    #39338
    Depositor
    SeWon Suh
    Insert
    Elongation factor 1 alpha-like protein
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  3. pCEP4_HLA-flag-GRM3 C127A-VN

    Plasmid
    #140449
    Purpose
    Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    FLAG tag, Signal/leader sequence from HLA class I…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    140449-plasmid-map-sequence-id-292293

    Enlarge

  4. pET28a hMDH1v3 (+TEV)

    Plasmid
    #204229
    Purpose
    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH1v3 (MDH1 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Inserted a G after ATG to maintain kozak site
    Promoter
    Lac
    Availability
    Academic Institutions and Nonprofits only
    204229-plasmid-map-sequence-id-413334

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  6. pTJK438

    Plasmid
    #138004
    Purpose
    3xMYC-SIX1 expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Tami Kingsbury
    Insert
    3xMYC tagged SIX1 (SIX1 Human)
    Use
    Lentiviral
    Tags
    3xMYC
    Expression
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    138004-plasmid-map-sequence-id-274998

    Enlarge

  7. ADH1pro_GCR2_SIR4

    Plasmid
    #138619
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GCR2 (GCR2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Availability
    Academic Institutions and Nonprofits only
    138619-plasmid-map-sequence-id-274988

    Enlarge

  8. ADH1pro_GAL4_SIR4

    Plasmid
    #138621
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GAL4 (GAL4 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Availability
    Academic Institutions and Nonprofits only
    138621-plasmid-map-sequence-id-334953

    Enlarge

  9. pcDNA3-Venus-cpVenus-FLARE-AKAR

    Plasmid
    #123329
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al Elife. 2018 Jul 3;7. pii: 35458
    Insert
    Venus-cpVenus-FLARE-AKAR
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    123329-plasmid-map-sequence-id-313436

    Enlarge

  11. pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

    Plasmid
    #123336
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al Elife. 2018 Jul 3;7. pii: 35458
    Insert
    Cerulean3-cpCerulean3-FLARE-AKAR
    Use
    Tags
    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    123336-plasmid-map-sequence-id-307044

    Enlarge

  12. pcDNA3-Venus-cpVenus-FLARE-CKAR

    Plasmid
    #123343
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al Elife. 2018 Jul 3;7. pii: 35458
    Insert
    Venus-cpVenus-FLARE-CKAR
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    123343-plasmid-map-sequence-id-307094

    Enlarge

Showing: 5021 - 5040 of 16077 results