Addgene

We narrowed to 34,820 results for: KAN

Showing: 5021 - 5040 of 34820 results

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pMis1_1B

Plasmid

#192079

Purpose

A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction

Depositor

Markus Buchhaupt

Article

Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.

Inserts

KanR
rhaR
rhaS
pBBR1 rep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-AKT

Plasmid

#20281

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

AKT (AKT1 Human)

Use

Transposon

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pLR5-CBh-dCas9-hUtx-IRES-Hyg

Plasmid

#122374

Purpose

Expresses dCas9-hUtx-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hUtx-IRES-Hyg-pA

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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OR6K6_Deletion_gRNA2

Plasmid

#195196

Purpose

dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR6K6 dual gRNA (OR6K6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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OR6K6_Deletion_gRNA1

Plasmid

#195195

Purpose

dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR6K6 dual gRNA (OR6K6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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OR6K6_Deletion_gRNA3

Plasmid

#195197

Purpose

dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR6K6 dual gRNA (OR6K6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OT1.0

Plasmid

#185387

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pNH605-HSE-YFP

Plasmid

#172131

Purpose

Fluorescent reporter for Hsf1 activity

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xHSE-YFP

Use

Vector for genomic integration into the yeast gen…

Tags

Expression

Yeast

Mutation

Promoter

CYC1 core promotor with 4 tandem repeats of the H…

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHS1862

Plasmid

#205975

Purpose

FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5 from JAGBLH010000220, Cas8-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1859

Plasmid

#205972

Purpose

FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Csy3/Cas7 from JAGBLH010000220, Cas8-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1860

Plasmid

#205973

Purpose

FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas6/Csy4 from JAGBLH010000220, Cas8-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pNH605-CCAAT-YFP

Plasmid

#172132

Purpose

Fluorescent reporter for HAP complex activity

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xCCAAT-YFP

Use

Vector for genomic integration into the yeast gen…

Tags

Expression

Yeast

Mutation

Promoter

CYC1 core promotor with 4 tandem repeats of the H…

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMM643

Plasmid

#112533

Purpose

pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA
luxCDABE

Use

Tags

Expression

Bacterial

Mutation

A17P

Promoter

J23107, PL(HrtO), and ProD

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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MSI2-ADA

Plasmid

#166967

Purpose

Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-ADA

Use

Retroviral

Tags

MSI2-ADA

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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HyperADAR control

Plasmid

#166969

Purpose

Express the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFP

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

linker-hyperADAR

Use

Retroviral

Tags

linker-HyperADAR

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM31

Plasmid

#52986

Purpose

A secreted Annexin V (sAnxV::GFP) Phosphatidylserine sensor

Depositor

Ding Xue

Article

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Annexin V (ANXA5 Human)

Use

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Mutation

Promoter

hsp-16.41

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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A2_uSFFV

Plasmid

#153415

Purpose

Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

A*02:01-P2A-human beta2 microglobulin (B2M Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHS1518

Plasmid

#205981

Purpose

FLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas6(Cse3/CasE) from MWEE01000013, Cas5-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHS1514

Plasmid

#205977

Purpose

FLAG-SV40 NLS-Cas8/Cse1 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8/Cse1 from MWEE01000013, Cas5-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1515

Plasmid

#205978

Purpose

FLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas11/Cse2 from MWEE01000013, Cas5-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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