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Showing: 5021 - 5040 of 30578 results

  2. pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)

    Plasmid
    #185488
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-P2A-eGFP and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185488-plasmid-map-sequence-id-362671

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  3. pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

    Plasmid
    #185485
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-P2A-eGFP and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185485-plasmid-map-sequence-id-362670

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  8. CA245 CMVTO_ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry

    Plasmid
    #231206
    Purpose
    Single transcript cageIL-10 (mRens) + cleaveIL-6 (mRens) + ER-renin (H2M)
    Depositor
    Xiaojing Gao
    Article
    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038
    Insert
    ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L147I, R148H, I203V, L290V
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    231206-plasmid-map-sequence-id-459396

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  10. pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro

    Plasmid
    #228399
    Purpose
    Encodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm
    Depositor
    Dirk Daelemans
    Article
    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:
    Insert
    3xFLAG-NLS/SV40-AcGFP-NES/PKI
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228399-plasmid-map-sequence-id-457131

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  11. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

    Plasmid
    #208290
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations and V106W in TadA and nSpCas9(D10…
    Promoter
    CMV and T7
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208290-plasmid-map-sequence-id-410848

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  14. SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)

    Plasmid
    #217801
    Purpose
    Variant CE1 construct with SaCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSaCas9(N580A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217801-plasmid-map-sequence-id-432878

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  15. Unfused click editor construct; mSA-ePhi29 - pCMV-T7-mSA-Phi29 (JO1411)

    Plasmid
    #217807
    Purpose
    Unfused click editor construct expressing mSA-ePhi29(D169A), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217807-plasmid-map-sequence-id-432906

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  16. HUHe variant click editor (CE1) - pCMV-T7-StCV-nCas9-EcKlenow (JO1269)

    Plasmid
    #217790
    Purpose
    Variant CE1 construct with StCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    StCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217790-plasmid-map-sequence-id-432853

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  17. HUHe variant click editor (CE1) - pCMV-T7-CaCV-nCas9-EcKlenow (JO1261)

    Plasmid
    #217791
    Purpose
    Variant CE1 construct with CaCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    CaCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217791-plasmid-map-sequence-id-432854

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  18. HUHe variant click editor (CE1) - pCMV-T7-Chimp17-nCas9-EcKlenow (JO1275)

    Plasmid
    #217792
    Purpose
    Variant CE1 construct with Chim HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Chimp17-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217792-plasmid-map-sequence-id-432856

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  19. HUHe variant click editor (CE1) - pCMV-T7-GoCV-nCas9-EcKlenow (JO1277)

    Plasmid
    #217793
    Purpose
    Variant CE1 construct with GoCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    GoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217793-plasmid-map-sequence-id-432858

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  20. HUHe variant click editor (CE1) - pCMV-T7-SwCV-nCas9-EcKlenow (JO1270)

    Plasmid
    #217794
    Purpose
    Variant CE1 construct with SwCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    SwCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217794-plasmid-map-sequence-id-432859

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Showing: 5021 - 5040 of 30578 results