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Plasmid#221939PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationTALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…PromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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FusX alphaDdCBE DddA11 1397N
Plasmid#221940PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA11 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationTALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…PromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX DdCBE 1397C
Plasmid#221931PurposeBackbone plasmid for FusX-based assembly of DdCBE arm with DddAtox 1397CDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX DdCBE 1397N
Plasmid#221932PurposeBackbone plasmid for FusX-based assembly of DdCBE arm with DddAtox 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX DdCBE DddA6-11 1397C
Plasmid#221933PurposeBackbone plasmid for FusX-based assembly of DdCBE arm with DddA6/11 1397CDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationDddA: T1413IPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Luciferase shRNA
Plasmid#225343PurposeshRNAmir backbone for RNA interference, with YFPDepositorInsertLuciferase shRNA (LOC116160065 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterCBAAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bacteroidetes-bGSDM-TEV
Plasmid#222101PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.DepositorInsertBacteroidetes-bGSDM-TEV ORF
UseTags6xHis-SUMO2ExpressionBacterialMutationTEV protease site replaces residues E242–G248PromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(2)-Gal4
Plasmid#220451PurposeSplit intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.DepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-TadA8e
Plasmid#209044PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLSDepositorInsertTadA8e-nSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(0)-Gal4DBD
Plasmid#220452PurposeSplit intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.DepositorInsertCfaN-Gal4DBD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(2)-Gal4DBD
Plasmid#220454PurposeSplit intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.DepositorInsertCfaN-Gal4DBD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(1)-p65AD
Plasmid#220456PurposeSplit intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.DepositorInsertCfaN-p65AD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(0)-Gal4
Plasmid#220449PurposeSplit intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.DepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(2)-LexAGAD
Plasmid#220460PurposeSplit intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.DepositorInsertCfaN-LexAGAD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(1)-Gal4DBD
Plasmid#220453PurposeSplit intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.DepositorInsertCfaN-Gal4DBD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(1)-LexAGAD
Plasmid#220459PurposeSplit intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.DepositorInsertCfaN-LexAGAD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(0)-p65AD
Plasmid#220455PurposeSplit intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.DepositorInsertCfaN-p65AD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(1)-Gal4
Plasmid#220450PurposeSplit intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.DepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(0)-LexAGAD
Plasmid#220458PurposeSplit intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.DepositorInsertCfaN-LexAGAD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(2)-p65AD
Plasmid#220457PurposeSplit intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.DepositorInsertCfaN-p65AD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only