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Plasmid#62445PurposeMXS_chaining vector with CMV::rtTA3-bGHpADepositorInsertcassette containing the reverse tetracycline transactivator with CMV promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-FLAG-mTUT4
Plasmid#60041PurposeMammalian expression of FLAG-tagged mouse TUT4DepositorInsertTUT4 (Tut4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo3
Plasmid#25103DepositorInsertpro-SUMO3 (SUMO3 Human)
UseTagsHisExpressionBacterialMutationchanged Serine 2 to AlaninePromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK1
Plasmid#118091PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 1
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME18S-3HA-mULK1
Plasmid#22896DepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
UseTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJZC518
Plasmid#62277PurposeYeast dCas9 expression plasmidDepositorInsertdCas9
UseTagsExpressionYeastMutationHas mutations in the RuvC1 and HNH domains to ren…PromoterpTdh3Available sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
MmSphk1
Plasmid#118599PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus sphingosine kinase 1
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::rtTA3-bGHpA
Plasmid#62446PurposeMXS_chaining vector with PGK::rtTA3-bGHpADepositorInsertcassette containing the reverse tetracycline transactivator with PGK promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTUT7
Plasmid#60044PurposeMammalian expression of FLAG-tagged mouse TUT7DepositorInsertTUT7 (Zcchc6 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo*
Plasmid#140159PurposeExpresses a regulatable secretory cargo (without an ER export signal) for mammalian cellsDepositorInsertpiGH-DsRed-Express2-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pTd-4-15-13-10
Plasmid#63835PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Col2.3deltaTK
Plasmid#114273PurposeConditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.DepositorInsertCol2.3 promoter + deltaTK + BGH polyA
UseTagsExpressionMammalianMutationPromoter2.3 kb rat type 1 collagenAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-002
Plasmid#78689PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::CreERT2-bGHpA
Plasmid#62444PurposeMXS_chaining vector with PGK::CreERT2-bGHpADepositorInsertcassette containing the tamoxifen inducible Cre recombinase with PGK Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Prohibitin-bio-His
Plasmid#50800PurposeExpresses enzymatically monobiotinylated full length ProhibitinDepositorInsertProhibitin
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
MXS_CMV::CreERT2-bGHpA
Plasmid#62443PurposeMXS_chaining vector with CMV::CreERT2-bGHpADepositorInsertcassette containing the tamoxifen inducible Cre recombinase with CMV Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME18S-3HA-mULK1(K46N)
Plasmid#22897DepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
UseTags3xHAExpressionMammalianMutationchanged Lysine 46 to AsparginePromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHL 246
Plasmid#53014PurposeAmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1DepositorInsertGFP
UseSynthetic BiologyTagsfimS (off orientation)ExpressionBacterialMutationPromoterAvailable sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -