Addgene

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pTrc-CysGA-Im7 F84A

Plasmid

#201082

Purpose

Im7 F84A variant inserted into CysGA at G364 via GS linker

Depositor

Shu Quan

Article

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Im7 F84A

Use

Tags

Expression

Bacterial

Mutation

Im7 F84A

Promoter

pTrc

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Green Falcan1

Plasmid

#199705

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pR

Plasmid

#193746

Purpose

Expresses LuxR under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-

Insert

LuxR

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23102

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-EGFP

Plasmid

#196606

Purpose

Dual-Luciferase Reporter vector carrying an EGFP direct sequence

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-SpDcp2opt_1-242_AD

Plasmid

#148547

Purpose

Bacterial Expression of SpDcp2opt_1-242

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp2opt_1-242 (dcp2 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-SpDCP1_X

Plasmid

#148003

Purpose

Bacterial Expression of SpDCP1

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDCP1 (dcp1 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-CtDCP2_1-267_X

Plasmid

#148004

Purpose

Bacterial Expression of CtDCP2_1-267

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

CtDCP2_1-267

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938-D603K_M

Plasmid

#146962

Purpose

Bacterial Expression of NcQde-2_506-938-D603K

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-D603K

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938-K638E_M

Plasmid

#146963

Purpose

Bacterial Expression of NcQde-2_506-938-K638E

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-K638E

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-A602E_M

Plasmid

#146964

Purpose

Bacterial Expression of NcQde-2_506-938-A602E

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-A602E

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-H609W_M

Plasmid

#146965

Purpose

Bacterial Expression of NcQde-2_506-938-H609W

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-H609W

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-K634A_M

Plasmid

#146966

Purpose

Bacterial Expression of NcQde-2_506-938-K634A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-K634A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-R895A_M

Plasmid

#146967

Purpose

Bacterial Expression of NcQde-2_506-938-R895A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-R895A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-Y595L_M

Plasmid

#146968

Purpose

Bacterial Expression of NcQde-2_506-938-Y595L

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-Y595L

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-SpDcp1opt_AD

Plasmid

#148538

Purpose

Bacterial Expression of SpDcp1opt

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp1opt (dcp1 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSMNot_sra-6::trp-4

Plasmid

#182261

Purpose

trp-4 cDNA cloned into pSMNot_sra-6 at Kpn&Notl

Depositor

Sreekanth Chalasani

Article

Ibsen et al Nat Commun. 2015 Sep 15;6:8264. doi: 10.

Inserts

trp-4 (trp-4 Nematode)
sra-6 (sra-6 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSMNot_odr-3::trp-4

Plasmid

#182262

Purpose

trp-4 cDNA cloned into pSMNot_odr-3 at KpnI & NotI

Depositor

Sreekanth Chalasani

Article

Ibsen et al Nat Commun. 2015 Sep 15;6:8264. doi: 10.

Inserts

trp-4 (trp-4 Nematode)
odr-3 (odr-3 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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BiDi-[mp-mEGFP]mp-mCherry2

Plasmid

#182870

Purpose

Bi-directional expression vector of monomers with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

Insert

myr-palm-mEGFP, myr-palm-mCherry2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-SpEdc1opt_155-180-GB1_AD

Plasmid

#148544

Purpose

Bacterial Expression of SpEDC1opt_155-180

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpEDC1opt_155-180

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#2

Plasmid

#163395

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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