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Plasmid#146732PurposeMammalian Expression of HseIF4EDepositorInsertHseIF4E (EIF4E Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0664
Plasmid#141655PurposeLentiviral vector for overexpressing the NKX1-2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNKX1-2 (NKX1-2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SUN1 GFP
Plasmid#187682PurposeIn vivo visualization of SUN1DepositorInsertSUN1 (Sun1 Mouse)
UseTagsEGFPExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGST2-TSG101 (2-145)
Plasmid#184810PurposeBacterial expression of TSG101 UEV domain (aa 2-145)DepositorInsertTSG101 (TSG101 Human)
UseTagsGSTExpressionBacterialMutationaa 2-145 onlyPromotertacAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_nocharge
Plasmid#139118Purposeexpress hnRNPA2 LC with no charged residuesDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall charged residues are changed to S/Q except fo…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_LacZ_sgRNA_2
Plasmid#155093Purposelentiviral plasmid expressing Cas9 and gRNA targeting LacZDepositorInsertLacZ_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 366-852)
Plasmid#119315Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 366-852 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 366-852PromoterCMVAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP
Plasmid#188777PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-bActinCDS-bActinUTR-MS2V5
Plasmid#102718PurposeHalo-Actin reporter for detection of both mRNA and proteinDepositorInsertHaloTag-bActinCDS-bActin3'UTR-MS2V5
UseLentiviralTagsHaloTag and MS2V5ExpressionMutationPromoterUbCAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
mcherry-DN KASH
Plasmid#125553Purposemcherry labeled DN-KASHDepositorInsertSYNE1 (SYNE1 Human)
UseTagsmcherryExpressionMammalianMutationTruncated form: only the KASH domain of nesprin-…PromoterCMVAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO EYFP (AAV9)
Viral Prep#55641-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Flp-dependent)Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n
Plasmid#46757DepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionBacterialMutationPromoterT3Available sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n
Plasmid#46757DepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionBacterialMutationPromoterT3Available sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin2
Plasmid#72595PurposeoverexpressionDepositorInsertSESN2 (SESN2 Human)
UseTagsFLAGExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin2
Plasmid#72595PurposeoverexpressionDepositorInsertSESN2 (SESN2 Human)
UseTagsFLAGExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pYPQ230-RRV
Plasmid#188543PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editingDepositorInsertLbCas12a-RRV
UseCRISPR; Gateway compatible lbcas12a-rrv entry clo…TagsNLSExpressionPlantMutationG146R, D156R, R182VPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgControl
Plasmid#125836PurposeKO controlDepositorInsertControl
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_MicroID2-HA
Plasmid#188521PurposeEncodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cellsDepositorInsertMicroID2
UseTransposon mediated gene integrationTagsHAExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only