Addgene

We narrowed to 3,077 results for: zebrafish

Showing: 481 - 500 of 3077 results

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pCSDest-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5

Plasmid

#192714

Purpose

Mammalian expression vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tag

Depositor

Summer Thyme

Insert

MCP-ADAR2-DD(E488Q/T490A)-V5

Use

Tags

V5

Expression

Mammalian

Mutation

E488Q/T490A

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

Plasmid

#67668

Purpose

Zebrafish expression vector for ubiquitous expression of APEX2-GBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pHis-zfCSF3b

Plasmid

#65613

Purpose

express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

Insert

Csf3b (csf3b Zebrafish)

Use

Tags

(His)6

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfTpo

Plasmid

#64309

Purpose

Express soluble His-tagged zebrafish thrombopoietin in insect cells

Depositor

Petr Bartunek

Article

Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

Insert

Thrombopoietin (thpo Zebrafish)

Use

Tags

(His)6

Expression

Insect

Mutation

Thrombopoietin starts at AA37 (AA1-36 encode the …

Promoter

Availability

Academic Institutions and Nonprofits only
pCS2-nCas9n-nanos 3’UTR

Plasmid

#62542

Purpose

expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

nanos 3' UTR (nanos3 Zebrafish)

Use

CRISPR

Tags

Expression

Mutation

3' UTR only

Promoter

SP6

Availability

Academic Institutions and Nonprofits only

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pTol2p2A--3mnx1:mTagBFP-NS-MCS

Plasmid

#78250

Purpose

Can be used to subclone in gene of interest into blue motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Inserts

-3mnx1 (mnx1 Zebrafish)
-mTagBFP-NS-MCS

Use

Zebrafish motor neuron expression

Tags

Expression

Mutation

Promoter

-3mnx1

Availability

Academic Institutions and Nonprofits only

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pCS2-GFP1-9

Plasmid

#124433

Purpose

Expresses GFP1-9 in zebrafish

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

GFP1-9

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-P2A-APEX2-VHH[GFP]

Plasmid

#126749

Purpose

Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

VHH[EGFP]

Use

Zebrafish muscle expression clone

Tags

mKate2-P2A-APEX2

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT3TS-zAsCpf1

Plasmid

#107185

Purpose

plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

AsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

Plasmid

#108871

Purpose

Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Inserts

5xU6:sgRNA
Cas9-t2A-GFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6 and hsp70

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj10a-IRES-EGFP

Plasmid

#164947

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj10a (kcnj10a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Expression

Mutation

Promoter

actinb (actb2) promoter

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcna1a-IRES-EGFP

Plasmid

#164965

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcna1a (kcna1a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Expression

Mutation

Promoter

actinb (actb2) promoter

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-MS2-Ds

Plasmid

#119076

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xMS2-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-com-Ds

Plasmid

#119077

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xCom-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfKitlga

Plasmid

#140292

Purpose

Express soluble His-tagged zebrafish Kitlga in insect cells

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlga (kitlga Zebrafish)

Use

Tags

6x His

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Cs2+Etv4(pea3)

Plasmid

#49093

Purpose

expression of Etv4

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv4 (etv4 Zebrafish)

Use

Xenopus/avian/zebrafish

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCS2+PhyBΔCreC

Plasmid

#131781

Purpose

Mammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC

Use

Cre/Lox; Zebrafish expression

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-Rab8

Plasmid

#194335

Purpose

Expresses FLAG tagged Rab8 in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Rab8 (RAB8A Canis lupus)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV IE94

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-Rab11

Plasmid

#194336

Purpose

Expresses FLAG tagged Rab11 in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Rab11 (RAB11A Rabbit)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV IE94

Availability

Academic Institutions and Nonprofits only

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