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Plasmid#215433PurposeExpression construct for UMG-SP-1 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-2_pET29b
Plasmid#215434PurposeExpression construct for UMG-SP-2 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL
Plasmid#26096PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHexahistidine tag with TEV cleavageExpressionBacterialMutationPromoterT7-lacOAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET51b(+)_EGFP_NbALFA
Plasmid#136626PurposePlasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-NbALFA
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delBRCT
Plasmid#173938PurposeBacterial expression of PARP1 lacking BRCT domainDepositorInsertPARP1delBRCT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 374-520; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delCAT
Plasmid#173942PurposeBacterial expression of PARP1 lacking CAT domainDepositorInsertPARP1delCAT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 661-1014PromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delWGR
Plasmid#173940PurposeBacterial expression of PARP1 lacking WGR domainDepositorInsertPARP1delWGR (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 521-660; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP1
Plasmid#128306PurposeExpress hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tagDepositorInsertHuman nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2
Plasmid#128307PurposeExpress hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tagDepositorInsertHuman Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
UseTagsHisExpressionBacterialMutationY85C- please see depositor comments belowPromoterT7Available sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIF
Plasmid#171082PurposeCo-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permissionDepositorUseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-C9ORF80
Plasmid#128418PurposeExpress C9ORF80 in E. coli with a His tag (C-terminal)DepositorInsertC9ORF80 (INIP Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29a-YS14
Plasmid#66890PurposePolycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)DepositorInsertsHis6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin
UseTagsH2A, H2B, H3, H4, His6 tag, and ThrombinExpressionBacterialMutationPromoterT7Available sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-28c S2
Plasmid#171920PurposeExpress RNA-based (p)ppGpp sensorDepositorInsertRNA-based (p)ppGpp sensor
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Npl4
Plasmid#117108PurposeProduction of recombinant Npl4 in E.coliDepositorInsertNpl4 (NPLOC4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP_PrA/G-H6
Plasmid#176077PurposeExpress His6 tagged MBP_PrA/G in Escherichia coliDepositorInsertMBP PrA/G-H6
UseTagsHis tagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FIP3
Plasmid#74753PurposeExpressed sfGFP-tagged FIP3 in E. coli for protein productionDepositorInsertRab11 family interacting protein 3 (RAB11FIP3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ube1/PET21d
Plasmid#34965DepositorInsertUbe1 (UBA1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
PET-3a-chpBK
Plasmid#155201PurposeTo express recombinant ChpBK proteinDepositorInsertschpS-chpBK-6Ă—His-Tag
GyrA-CBD-10xHis-Tag
UseTags10xHis and 6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RILP
Plasmid#74600Purposeused for expression in E. coli and purificationDepositorInsertRab-interacting lysosomal protein (RILP Human)
UseTagsFLAG, StrepII, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SrtAdelta59
Plasmid#51138Purposeexpression plasmid for N-terminal his tagged S.aureus SrtA delta 59DepositorInsertS.aureus SrtA delta 59
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only