Addgene

We narrowed to 2,412 results for: organization Addgene

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pLNCX2-mEGFP-VPS4A

Plasmid

#116924

Purpose

expresses mEGFP-VPS4A in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

VPS4A (VPS4A Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

silent mutation in position 4059 C to T

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-DO(FAS)-GCaMP6s

Plasmid

#110135

Purpose

Cre-off expression of GCaMP6s

Depositor

Bernardo Sabatini

Article

Markowitz et al Cell. 2018 May 10. pii: S0092-8674(18)30

Insert

GCaMP6s

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-4031NES

Plasmid

#105241

Purpose

A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

Depositor

Michiyuki Matsuda

Article

Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

Insert

AMPK-EV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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CTB

Plasmid

#141166

Purpose

Expresses recombinant Cholera Toxin B Subunit

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

CTB

Use

Tags

Expression

Bacterial

Mutation

No secretion signal

Promoter

T7

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-ABI-dCas9-P2A-mcherry

Plasmid

#121513

Purpose

expression of AB1-fused-dcas9 plasmids

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9

Use

Lentiviral

Tags

AB1 and P2A-mcherry

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+(Zeo)-FLAG-muGFP

Plasmid

#166726

Purpose

Expresses FLAG-tagged monomeric ultrastable GFP (muGFP) in mammalian cells

Depositor

Daniel Scott

Article

Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

Insert

muGFP

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSEMS-Halo7Tag-hFis

Plasmid

#111136

Purpose

hFis with selflabeling tag, e.g. for fluorescence microscopy

Depositor

Karin Busch

Article

Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

Insert

human fission factor1 (FIS1 Human)

Use

Tags

Halo7-tag

Expression

Mammalian

Mutation

*

Promoter

CMV

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pJJR83

Plasmid

#75028

Purpose

mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

mCherry^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized mCherry

Expression

Worm

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAV5B+longBRD4

Plasmid

#157792

Purpose

Expression of long isoform of BRD4

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

long BRD4 (BRD4 Human)

Use

Tags

His6

Expression

Insect

Mutation

Promoter

polyhedron

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+H1.4

Plasmid

#157796

Purpose

Expression of human linker histone H1.4

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H1.4 (H1-4 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSEMS-Tom20-Halo7Tag

Plasmid

#111135

Purpose

Tom20 with selflabeling tag, e.g. for fluorescence microscopy

Depositor

Karin Busch

Article

Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

Insert

Receptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)

Use

Tags

Halo7 Tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pJJR82

Plasmid

#75027

Purpose

EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

EGFP^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized EGFP

Expression

Worm

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAM-DCA-muGFP

Plasmid

#166727

Purpose

AAV, encodes monomeric ultrastable GFP (muGFP)

Depositor

Daniel Scott

Article

Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

Insert

muGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Chicken Beta Actin

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

Plasmid

#179586

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NeonGreen

Expression

Mutation

Promoter

TRE

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-Lifeact-mRuby

Plasmid

#194339

Purpose

Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Lifeact

Use

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Cdc42BPB

Plasmid

#50759

Purpose

expression of EGFP tagged Cdc42BPB

Depositor

Naoki Mochizuki

Article

Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

Insert

Cdc42BPB (CDC42BPB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pETduet+sfGFP-TetR-p300HAT

Plasmid

#157795

Purpose

Expression of sfGFP-TetR-p300HAT fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

sfGFP-TetR-p300 (EP300 Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO CBX1-APEX2

Plasmid

#129642

Purpose

Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

CBX1 (CBX1 Human)

Use

Tags

1xFLAG and APEX2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-mRuby-Rab8a

Plasmid

#194337

Purpose

Expresses mRuby Rab8a in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Rab8 (RAB8A Canis lupus)

Use

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pUBC_HA_PP7-mVenus

Plasmid

#111268

Purpose

Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

Depositor

John Rinn

Article

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

PP7-mVenus

Use

Synthetic Biology

Tags

HA, SV40 NLS, and mVenus

Expression

Mammalian

Mutation

PP7: C68S, S69G [delta]70-75 (non-aggregating)

Promoter

hUBC

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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