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Plasmid#169575PurposeTier-1 vector encoding PhCMV-driven tetracycline-dependent VPR-based transactiavtor (PhCMV-TetR-VPR-pA).DepositorInserttetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB301-Tier1-PhCMV-rTetR_VPR
Plasmid#169579PurposeTier-1 vector encoding PhCMV-driven tetracycline-activated VPR-based transactiavtor (PhCMV-rTetR-VPR-pA).DepositorInsertreverse tetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH227-Tier1-OTetO7-PCMVmin2-SEAP
Plasmid#169563PurposeTier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).DepositorInserttetracycline-controlled SEAP production
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVminAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pChlr-7SE
Plasmid#52168PurposeEncodes module 7 with amino acids 12S-16E for recognition of nt 2GDepositorInsertPumilio homology domain amino acids 241-283 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFOX13-Tier1-PhCMV-tGFP
Plasmid#169510PurposeTier-1 vector encoding PhCMV-driven green fluorescent protein turbo GFP (PhCMV-tGFP-pA).DepositorInsertPCMV-driven turbo green fluorescent protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas2D
Plasmid#82375PurposedCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Prha-base
Plasmid#169244PurposeSuicide vector for integrating expression cassette in P. putida (L-rhamnose induction)DepositorInsertrhaRS
UseTagsExpressionBacterialMutationPromoterrhaSAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4SYE
Plasmid#52156PurposeEncodes module 4 with amino acids 12S-13Y-16E for recognition of nt 5GDepositorInsertD43951 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S, 13H to 13Y, and 16Q to 16E in…PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-3CQ
Plasmid#52147PurposeEncodes module 3 with amino acids 12C-16Q for recognition of nt 6ADepositorInsertPumilio homology domain amino acids 97-132 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-1SQ
Plasmid#52139PurposeEncodes module 1 with amino acids 12S-16Q for recognition of nt 8ADepositorInsertPumilio homology domain amino acids 25-60 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2NQ
Plasmid#52145PurposeEncodes module 2 with amino acids 12N-16Q for recognition of nt 7UDepositorInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4NQ
Plasmid#52153PurposeEncodes module 4 with amino acids 12N-16Q for recognition of nt 5UDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5CQ
Plasmid#52159PurposeEncodes module 5 with amino acids 12C-16Q for recognition of nt 4ADepositorInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-6NQ
Plasmid#52165PurposeEncodes module 6 with amino acids 12N-16Q for recognition of nt 3UDepositorInsertPumilio homology domain amino acids 205-240 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-8NQ
Plasmid#52173PurposeEncodes module 8 with amino acids 12N-16Q for recognition of nt 1UDepositorInsertPumilio homology domain amino acids 284-319 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas61
Plasmid#82397PurposeIPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpKDepositorInsertLDH
UseTagsExpressionMutationV39RPromotercLac143Available sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
UseTagsMaltose binding protein and His tagExpressionBacterialMutationPromoterVhbAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-pVhb-tdTER-fdh
Plasmid#215649PurposepBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoterDepositorInsertstrans-enoyl-CoA reductase
formate dehydrogenase
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-MET-IV
Plasmid#218117PurposeMET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only