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Plasmid#115249PurposeFor mammalian expression of a human NODAL splice variant open reading frame with an internal MYC tagDepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-T7TS
Plasmid#115251PurposeFor in vitro transcription of a human NODAL splice variant open reading frame RNADepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmKaxxte
Plasmid#113630Purposeto test Cas9 gRNA efficiency or HR-capacityDepositorInsertmKaxxte2.5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYSD085
Plasmid#212922PurposeEncodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsert649 Stalk
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJG100
Plasmid#138966PurposePeft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegansDepositorInsertwrmScarlet1-10
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDAS12125_PEAR-GFP
Plasmid#177178PurposePEAR-GFP target plasmid with split EGFP and disrupted 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHW533 (Peft-3::cGAL-N::let-858 3'UTR)
Plasmid#107132PurposecGAL-N driver under the control of C. elegans eft-3 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans eft-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW531 (Peft-3::cGAL-C::let-858 3'UTR)
Plasmid#107136PurposecGAL-C driver under the control of C. elegans eft-3 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
UseTagsExpressionWormMutationPromoterC. elegans eft-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS423-GPD-wrmScarlet1-10-CYC1 terminator
Plasmid#158584PurposeExpresses wrmScarlet1-10 in S. cerevisiaeDepositorInsertwrmScarlet1-10
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHW530 (Prab-3::cGAL-N::let-858 3'UTR)
Plasmid#107131PurposecGAL-N driver under the control of C. elegans rab-3 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans rab-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW522 (Prab-3::cGAL-C::let-858 3'UTR)
Plasmid#107130PurposecGAL-C driver under the control of C. elegans rab-3 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
UseTagsExpressionWormMutationPromoterC. elegans rab-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAH36 (Pmyo-2::cGAL-C::let-858 3'UTR)
Plasmid#107135PurposecGAL-C driver under the control of C. elegans myo-2 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
UseTagsExpressionWormMutationPromoterC. elegans myo-2 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CTE-Luc
Plasmid#60881PurposeReporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTEDepositorInsertLuc-CTE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAH35 (Pmyo-2::cGAL-N::let-858 3'UTR)
Plasmid#107134PurposecGAL-N driver under the control of C. elegans myo-2 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans myo-2 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top-Toehold-mCherry
Plasmid#87318PurposeExpresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.DepositorInsertSplit-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T~Bottom-T
Plasmid#87315PurposeControl plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.DepositorInsertSplit-Broccoli system (promoter control)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHYDuet93
Plasmid#42303DepositorInsertNpuDnaEdC6
UseTagsH6-GB1ExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSuper-TatSF1 GH
Plasmid#31120DepositorInsertTatSF1 shRNA (HTATSF1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSuper-TatSF1 AB
Plasmid#31119DepositorInsertTatSF1 shRNA (HTATSF1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
GST-beta catenin tag
Plasmid#186422PurposeEncodes GST fused to beta catenin tag recognized by anti-beta catenin nanobodyDepositorInsertGST-beta catenin tag
UseTags6His and GST-beta catenin tag (PDRKAAVSHWQQRS)ExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only