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Plasmid#145420PurposeYeast Expression plasmid for SARS-CoV-2 nsp11DepositorInsertSARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046321
Plasmid#145409PurposeYeast Expression plasmid for SARS-CoV-2 nsp11DepositorInsertSARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;MBPExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTP1005
Plasmid#104160PurposeBacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)DepositorInsertAnti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PCMV-flag-Caspase11
Plasmid#21145DepositorInsertCaspase11 (Casp4 Mouse)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFG036 + pFG051
Plasmid#137997PurposeTransposon mutagenesis plasmid and the cI repressor expression plasmidDepositorInsertTn5 tnp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
PEP109E
Plasmid#97395Purposebinary construct of cytosolic mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagscytosolExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIK3CD
Plasmid#174190PurposeExpresses PIK3CD in mammalian cellsDepositorInsertPIK3CD (PIK3CD Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycMOV10
Plasmid#19875DepositorInsertMoloney leukemia virus 10, homolog (MOV10 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
HLTF
Plasmid#28152PurposeBacterial expression for structure determination; may not be full ORFDepositorInserthelicase-like transcription factor (HLTF Human)
UseTagsthrombin cleavageExpressionBacterialMutationContains HIRAN domain (residues 51 to 171).PromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
1BR9
Plasmid#193862PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged proteins. Empty backbone for LIC cloning.DepositorTypeEmpty backboneUseTagsGFP11, Hisx6, and R9ExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas9_CL
Plasmid#166245Purposeregulated dCas9 generatorDepositorInsertP112-sgRNA-term-P104-RBS1-dCas9-B15
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterEc-TTL-P112 and Ec-TTL-P104Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAUX_OL
Plasmid#166246Purposeuse as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmidDepositorInsert[P112-sgRNA-term]-[J23116_B34-dCas9-B15]
UseTagsExpressionBacterialMutationPromoterEc-TTL-P112 and BBa_J23116Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMKO.1 puro Rb shRNA
Plasmid#10670DepositorInsertRb shRNA (RB1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Sec16B
Plasmid#66607PurposeExpresses Sec16B as a GFP fusionDepositorInsertSec16B (SEC16B Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
1BR9-mCherry
Plasmid#193864PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged mCherry.DepositorInsertmCherry
UseTags6xHis, GFP11, and R9ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
1BR9-mCherrySTOP
Plasmid#193866PurposeE. coli expression vector for N-ter GFP11 tagged mCherry.DepositorInsertmCherry
UseTags6xHis and GFP11ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAP1638
Plasmid#137089PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)DepositorInsert4*(GFP11)
UsePcr template for donor dnaTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-RB (829-928)
Plasmid#31498DepositorInsertRB (RB1 Human)
UseTagsGSTExpressionBacterialMutationContains amino acids 829-928PromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
GST-RB (736-840)
Plasmid#31497DepositorInsertRB (RB1 Human)
UseTagsGSTExpressionBacterialMutationContains amino acids 736-840PromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only