-
Plasmid#198105Purposelentiviral expression of hCOL4A5_C1567R in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseLentiviralTagsHAExpressionMutationC1567RPromoterEF1aAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Blast_hCOL4A5-HA_G869R
Plasmid#198104Purposelentiviral expression of hCOL4A5_G869R in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseLentiviralTagsHAExpressionMutationG869RPromoterEF1aAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-LASSIE
Plasmid#192105PurposeLentiviral vector expressing long non-coding RNA LASSIE (linc00520)DepositorInsertLASSIE
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast
Plasmid#182887PurposeTransfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)DepositorInsertHAUS 6 (HAUS6 Human)
UseLentiviralTagsEGFPExpressionBacterial and MammalianMutationsilet mutations in aa 197 - 203 to confer resista…PromoterEF1alpha coreAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-SpRYgest_targetplasmid (KAC833)
Plasmid#181748PurposepUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.DepositorTypeEmpty backboneUseSubstrate plasmidTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.Ollas.V5_mCherry-NLS
Plasmid#178233PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.Ollas.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.Ollas.VSVg_mCherry-NLS
Plasmid#178232PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.Ollas.VSVg and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.Ollas.S_mCherry-NLS
Plasmid#178231PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.Ollas.S and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.NWS.Ollas_mCherry-NLS
Plasmid#178227PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.NWS.Ollas and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lentiguide-Blast-eNMU710
Plasmid#172658PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locusDepositorInsertpegRNA-eNMUA/pegRNA-eNMUB
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Negative feedback (NF) plasmid
Plasmid#169747PurposeStandalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.DepositorInserttetR-P2A-eGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-CTSS-blast
Plasmid#158470PurposeConstitutive lentiviral expression of human CTSS.DepositorInsertCTSS (CTSS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgMARK3
Plasmid#138706PurposeExpresses a human MARK3-targeting sgRNA and Cas9DepositorInsertsgMARK3 human (MARK3 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgMARK4
Plasmid#138707PurposeExpresses a human MARK4-targeting sgRNA and Cas9DepositorInsertsgMARK4 human (MARK4 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgSIK1-D
Plasmid#138680PurposeExpresses a mouse SIK1-targeting sgRNA and Cas9DepositorInsertsgSIK1 mouse (Sik1 Mouse)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgSIK1-B
Plasmid#138679PurposeExpresses a mouse SIK1-targeting sgRNA and Cas9DepositorInsertsgSIK1 mouse (Sik1 Mouse)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgBRSK1
Plasmid#138705PurposeExpresses a human BRSK1-targeting sgRNA and Cas9DepositorInsertsgBRSK1 human (BRSK1 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_mTq-Tat (c-plasmid)
Plasmid#127635PurposeEncodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.DepositorInsertmTurquoise2
UseSynthetic BiologyTagsRNA binding peptide: PCP and RNA binding peptide:…ExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 PCSK5-V5 blast
Plasmid#83100PurposeLentiviral vector for constitutive expression of human PC5A (PCSK5) with C-terminal tagDepositorInsertPCSK5 (PCSK5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
PE_22: Plas_tet_eG::sfYFP
Plasmid#104883PurposePE_22: Plas_tet_eG::sfYFPDepositorInsertHybrid Promoter: Plas_tet_eG
UseTagsExpressionBacterialMutationPromoterHybrid Promoter : Plas_tet_eGAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only