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Plasmid#178957PurposeIt consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.DepositorInsertSpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMB28
Plasmid#223011PurposeCas12a and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assayDepositorInsertCas12a and Csy4 with 5' and 3' targets (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
SunTagCACTA1g2-22aa-TET1cd
Plasmid#106437PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoterDepositorInsertCACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB109
Plasmid#223017PurposeCas9 and Csy4 with 5' and 3' targets (II) for Lotus callus assayDepositorInsertCas9 and Csy4 with 5' and 3' targets (II)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB20
Plasmid#223004PurposeCsy4 with 5' and 3' targets (I) and without Cas for Lotus callus assayDepositorInsertCsy4 with 5' and 3' targets (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB21
Plasmid#223005PurposeCsy4 with 5' and 3' targets (II) and without Cas for Lotus callus assayDepositorInsertCsy4 with 5' and 3' targets (II)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB22
Plasmid#223006PurposeControl plasmid without Cas and Csy4 for Lotus callus assayDepositorInsertNot applicable, control plasmid
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB23
Plasmid#223007PurposeCsy4 with 5' and 3' targets (I) and without Cas for Nicotiana leaf assayDepositorInsertCsy4 with 5' and 3' targets (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB24
Plasmid#223008PurposeControl plasmid without Cas and Csy4 for Nicotiana leaf assayDepositorInsertNot applicable, control plasmid
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB26
Plasmid#223009PurposeCas12a and Csy4 with 5' target (I) for Nicotiana leaf assayDepositorInsertCas12a and Csy4 with 5' target (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB27
Plasmid#223010PurposeCas12a and Csy4 with 3' target (I) for Nicotiana leaf assayDepositorInsertCas12a and Csy4 with 3' target (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB45
Plasmid#223012PurposeCas12a and Csy4 with 5' and 3' targets (I) for Lotus callus assayDepositorInsertCas12a and Csy4 with 5' and 3' targets (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB51
Plasmid#223013PurposeCas12a and Csy4 with 5' and 3' targets (II) for Lotus callus assayDepositorInsertCas12a and Csy4 with 5' and 3' targets (II)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB61
Plasmid#223014PurposeCas12a and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assayDepositorInsertCas12a and Csy4 with 5' and 3' targets (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB105
Plasmid#223016PurposeCas9 and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assayDepositorInsertCas9 and Csy4 with 5' and 3' targets (I)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11
Plasmid#211942Purposedual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterHSP68Available sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-BsaI_pJMP2776
Plasmid#208890PurposeAcinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).DepositorInsertdCas9, sgRNA expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNTI743 dual-barcoded gRNA parent vector
Plasmid#164915PurposeCEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid librariesDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI733 pRPR1(TetO)-RPC31-sgRNA
Plasmid#164913PurposeFor yeast genomic integration of sgRNA against RPC31DepositorInsertpRPR1(TetO)-RPC31-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterRPR1Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI732 pRPR1(TetO)-HTS1-sgRNA
Plasmid#164912PurposeFor yeast genomic integration of sgRNA against HTS1DepositorInsertpRPR1(TetO)-HTS1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterRPR1Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only