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Showing: 481 - 500 of 9659 results

  1. PET.SUMO eIF4G1 +MIC 1-200

    Plasmid
    #158792
    Purpose
    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1 (with microexon) codon optimized
    Use
    Tags
    6xHIS and SUMO
    Expression
    Bacterial
    Mutation
    amino acids 1-200
    Promoter
    T7
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158792-plasmid-map-sequence-id-309816

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  2. p663-UBC-miniTurbo-V5-RIOK3_IDG-K

    Plasmid
    #158188
    Purpose
    Gateway destination clone of RIOK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-RIOK3 (RIOK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    158188-plasmid-map-sequence-id-314664

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  3. p663-UBC-miniTurbo-V5-CDK16_IDG-K

    Plasmid
    #158173
    Purpose
    Gateway destination clone of CDK16 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK16 (CDK16 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158173-plasmid-map-sequence-id-309975

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  6. p663-UBC-miniTurbo-V5-DSTYK _IDG-K

    Plasmid
    #158182
    Purpose
    Gateway destination clone of DSTYK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-DSTYK (DSTYK Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    158182-plasmid-map-sequence-id-314617

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  8. pLKO_HA.AU1.VSVg_NGFR

    Plasmid
    #158232
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.AU1.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158232-plasmid-map-sequence-id-334981

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  9. human SLC12A5-V5-HA-2A-Luciferase repair template

    Plasmid
    #158576
    Purpose
    For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.
    Depositor
    Rudolf Jaenisch
    Article
    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii
    Insert
    human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
    Use
    Tags
    V5 and HA on the endogenous KCC2 protein product
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    158576-plasmid-map-sequence-id-311917

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  10. pLEX307-CTSL-blast

    Plasmid
    #158467
    Purpose
    Constitutive lentiviral expression of human CTSL.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSL (CTSL Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158467-plasmid-map-sequence-id-308152

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  11. pZA1

    Plasmid
    #158479
    Purpose
    Entry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1 (ZAR1 Mustard Weed)
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    STOP codon
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    158479-plasmid-map-sequence-id-309695

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  14. pLEX307-CTSS-G418

    Plasmid
    #158471
    Purpose
    Constitutive lentiviral expression of human CTSS.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSS (CTSS Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158471-plasmid-map-sequence-id-308129

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  18. L3670

    Plasmid
    #1586
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    let858Tsa, ATG-MitLS, gfpN, IVSR, gfpS65C, IVSS, …
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1586-plasmid-map-sequence-id-196

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  20. p663-UBC-miniTurbo-V5-VRK2_IDG-K

    Plasmid
    #158196
    Purpose
    Gateway destination clone of VRK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-VRK2 (VRK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    158196-plasmid-map-sequence-id-314666

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Showing: 481 - 500 of 9659 results